

Recombinant Anti-QPRT antibody [EPR11941(B)] (ab171944)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-QPRT antibody [EPR11941(B)]
See all QPRT primary antibodies -
Description
Rabbit monoclonal [EPR11941(B)] to QPRT -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human QPRT aa 250 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q15274 -
Positive control
- Human fetal kidney, Human fetal liver and Human fetal brain lysate, HepG2 and HeLa cell line lysates, Jurkat cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11941(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab171944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/5000. Predicted molecular weight: 31 kDa. | |
Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Involved in the catabolism of quinolinic acid (QA). -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1. -
Sequence similarities
Belongs to the nadC/modD family. - Information by UniProt
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Database links
- Entrez Gene: 23475 Human
- Omim: 606248 Human
- SwissProt: Q15274 Human
- Unigene: 513484 Human
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Alternative names
- NADC_HUMAN antibody
- Nicotinate nucleotide pyrophosphorylase (carboxylating) antibody
- Nicotinate-nucleotide pyrophosphorylase [carboxylating] antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: QPRT knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Human fetal brain cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HepG2 cell line lysate
Lane 4 : HeLa cell line lysate
Lane 5 : Jurkat cell line lysate
Lane 6 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa -
Flow cytometric analysis of permeabilized Jurkat cells labeling QPRT with ab171944 at 1/10 (red) or a rabbit IgG (negative) (green).
Datasheets and documents
References
ab171944 has not yet been referenced specifically in any publications.