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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-QPRT antibody [EPR11941(B)] (ab171944)

Submit a review Submit a question References (4)
Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
  • Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
  • Flow Cytometry (Intracellular) - Anti-QPRT antibody [EPR11941(B)] (ab171944)
  • Anti-QPRT antibody [EPR11941(B)] (ab171944)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11941(B)] to QPRT
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-QPRT antibody [EPR11941(B)]
    See all QPRT primary antibodies

  • Description

    Rabbit monoclonal [EPR11941(B)] to QPRT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human QPRT aa 250 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15274

  • Positive control

    • Human fetal kidney, Human fetal liver and Human fetal brain lysate, HepG2 and HeLa cell line lysates, Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
Application notes
Is unsuitable for ICC/IF,IHC or IP.

Target

  • Function

    Involved in the catabolism of quinolinic acid (QA).

  • Pathway

    Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.

  • Sequence similarities

    Belongs to the nadC/modD family.
  • Information by UniProt

  • Database links

    • Alternative names

      • NADC_HUMAN antibody
      • Nicotinate nucleotide pyrophosphorylase (carboxylating) antibody
      • Nicotinate-nucleotide pyrophosphorylase [carboxylating] antibody
      • QAPRTase antibody
      • QPRT antibody
      • QPRTase antibody
      • Quinolinate phosphoribosyltransferase [decarboxylating] antibody
      • Quinolinate phosphoribosyltransferase antibody
      see all

    Images

    • Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
      Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: QPRT knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: Human fetal brain cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
      Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
      All lanes : Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Human fetal liver tissue lysate
      Lane 3 : HepG2 cell line lysate
      Lane 4 : HeLa cell line lysate
      Lane 5 : Jurkat cell line lysate
      Lane 6 : Human fetal heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 31 kDa

    • Flow Cytometry (Intracellular) - Anti-QPRT antibody [EPR11941(B)] (ab171944)
      Flow Cytometry (Intracellular) - Anti-QPRT antibody [EPR11941(B)] (ab171944)

      Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling QPRTwith ab171944 at 1/10 (red)or a rabbit IgG (negative) (green). 

       

       

    • Anti-QPRT antibody [EPR11941(B)] (ab171944)
      Anti-QPRT antibody [EPR11941(B)] (ab171944)

    References (4)

    Publishing research using ab171944? Please let us know so that we can cite the reference in this datasheet.

    ab171944 has been referenced in 4 publications.

    • Zhou XY  et al. Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney. Front Genet 12:575830 (2021). PubMed: 34079576
    • Kudo K  et al. Divergent metabolic responses dictate vulnerability to NAMPT inhibition in ovarian cancer. FEBS Lett 594:1379-1388 (2020). PubMed: 31950503
    • Niu YC  et al. MicroRNA-654-3p enhances cisplatin sensitivity by targeting QPRT and inhibiting the PI3K/AKT signaling pathway in ovarian cancer cells. Exp Ther Med 20:1467-1479 (2020). PubMed: 32742380
    • Yue Z  et al. Silencing DSCAM-AS1 suppresses the growth and invasion of ER-positive breast cancer cells by downregulating both DCTPP1 and QPRT. Aging (Albany NY) 12:14754-14774 (2020). PubMed: 32716908

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