Validated using a knockout cell line
Recombinant
RabMAb

Anti-QPRT antibody [EPR11941(B)] (ab171944)

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Overview

  • Product name
    Anti-QPRT antibody [EPR11941(B)]
  • Description
    Rabbit monoclonal [EPR11941(B)] to QPRT
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human QPRT aa 250 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15274

  • Positive control
    • Human fetal kidney, Human fetal liver and Human fetal brain lysate, HepG2 and HeLa cell line lysates, Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC or IP.

    • Target

    • Function
      Involved in the catabolism of quinolinic acid (QA).
    • Pathway
      Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.
    • Sequence similarities
      Belongs to the nadC/modD family.
    • Information by UniProt
    • Database links
      • Alternative names
        • NADC_HUMAN antibody
        • Nicotinate nucleotide pyrophosphorylase (carboxylating) antibody
        • Nicotinate-nucleotide pyrophosphorylase [carboxylating] antibody
        • QAPRTase antibody
        • QPRT antibody
        • QPRTase antibody
        • Quinolinate phosphoribosyltransferase [decarboxylating] antibody
        • Quinolinate phosphoribosyltransferase antibody
        see all

      Images

      • Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
        Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: QPRT knockout HAP1 cell lysate (20 µg)
        Lane 3: HepG2 cell lysate (20 µg)
        Lane 4: Human fetal brain cell lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

      • Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
        Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
        All lanes : Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution

        Lane 1 : Human fetal kidney tissue lysate
        Lane 2 : Human fetal liver tissue lysate
        Lane 3 : HepG2 cell line lysate
        Lane 4 : HeLa cell line lysate
        Lane 5 : Jurkat cell line lysate
        Lane 6 : Human fetal heart tissue lysate

        Lysates/proteins at 10 µg per lane.

        Developed using the ECL technique.

        Predicted band size: 31 kDa

      • Flow Cytometry - Anti-QPRT antibody [EPR11941(B)] (ab171944)
        Flow Cytometry - Anti-QPRT antibody [EPR11941(B)] (ab171944)

        Flow cytometric analysis of permeabilized Jurkat cells labeling QPRT with ab171944 at 1/10 (red) or a rabbit IgG (negative) (green).

      References

      ab171944 has not yet been referenced specifically in any publications.

      Publishing research using ab171944? Please let us know so that we can cite the reference in this datasheet.

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