Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-QPRT antibody [EPR11941(B)] (ab171944)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11941(B)] to QPRT
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-QPRT antibody [EPR11941(B)]
    See all QPRT primary antibodies

  • Description

    Rabbit monoclonal [EPR11941(B)] to QPRT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human QPRT aa 250 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15274

  • Positive control

    • Human fetal kidney, Human fetal liver and Human fetal brain lysate, HepG2 and HeLa cell line lysates, Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab171944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC or IP.

    • Target

    • Function

      Involved in the catabolism of quinolinic acid (QA).

    • Pathway

      Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.

    • Sequence similarities

      Belongs to the nadC/modD family.
    • Information by UniProt

    • Database links

      • Alternative names

        • NADC_HUMAN antibody
        • Nicotinate nucleotide pyrophosphorylase (carboxylating) antibody
        • Nicotinate-nucleotide pyrophosphorylase [carboxylating] antibody
        • QAPRTase antibody
        • QPRT antibody
        • QPRTase antibody
        • Quinolinate phosphoribosyltransferase [decarboxylating] antibody
        • Quinolinate phosphoribosyltransferase antibody
        see all

      Images

      • Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
        Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)

        Lane 1: Wild-type HAP1 cell lysate (20 µg)
        Lane 2: QPRT knockout HAP1 cell lysate (20 µg)
        Lane 3: HepG2 cell lysate (20 µg)
        Lane 4: Human fetal brain cell lysate (20 µg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

        ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

      • Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
        Western blot - Anti-QPRT antibody [EPR11941(B)] (ab171944)
        All lanes : Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution

        Lane 1 : Human fetal kidney tissue lysate
        Lane 2 : Human fetal liver tissue lysate
        Lane 3 : HepG2 cell line lysate
        Lane 4 : HeLa cell line lysate
        Lane 5 : Jurkat cell line lysate
        Lane 6 : Human fetal heart tissue lysate

        Lysates/proteins at 10 µg per lane.

        Developed using the ECL technique.

        Predicted band size: 31 kDa

      • Flow Cytometry - Anti-QPRT antibody [EPR11941(B)] (ab171944)
        Flow Cytometry - Anti-QPRT antibody [EPR11941(B)] (ab171944)

        Flow cytometric analysis of permeabilized Jurkat cells labeling QPRT with ab171944 at 1/10 (red) or a rabbit IgG (negative) (green).

      • Anti-QPRT antibody [EPR11941(B)] (ab171944)
        Anti-QPRT antibody [EPR11941(B)] (ab171944)

      References (0)

      Publishing research using ab171944? Please let us know so that we can cite the reference in this datasheet.

      ab171944 has not yet been referenced specifically in any publications.

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