Product nameAnti-QPRT antibody [EPR11941(B)]
See all QPRT primary antibodies
DescriptionRabbit monoclonal [EPR11941(B)] to QPRT
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human QPRT aa 250 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q15274
- Human fetal kidney, Human fetal liver and Human fetal brain lysate, HepG2 and HeLa cell line lysates, Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 31 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the catabolism of quinolinic acid (QA).
PathwayCofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.
Sequence similaritiesBelongs to the nadC/modD family.
- Information by UniProt
- NADC_HUMAN antibody
- Nicotinate nucleotide pyrophosphorylase (carboxylating) antibody
- Nicotinate-nucleotide pyrophosphorylase [carboxylating] antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: QPRT knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Human fetal brain cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HepG2 cell line lysate
Lane 4 : HeLa cell line lysate
Lane 5 : Jurkat cell line lysate
Lane 6 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa
Flow cytometric analysis of permeabilized Jurkat cells labeling QPRT with ab171944 at 1/10 (red) or a rabbit IgG (negative) (green).
ab171944 has not yet been referenced specifically in any publications.