Key features and details
- Rabbit polyclonal to QSOX2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-QSOX2 antibody
See all QSOX2 primary antibodies
DescriptionRabbit polyclonal to QSOX2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
internal sequence amino acids 504-633 of Human QSOX2.
- Human epididymis tissue; RT4 and U-251 MG cell lysate; Human Plasma.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionCatalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. Also seems to play a role in regulating the sensitization of neuroblastoma cells for interferon-gamma-induced apoptosis.
Tissue specificityExpressed in pancreas, brain, placenta, kidney, heart and fetal tissues. Weakly expressed in lung, liver and skeletal muscles.
Sequence similaritiesBelongs to the quiescin-sulfhydryl oxidase (QSOX) family.
Contains 1 ERV/ALR sulfhydryl oxidase domain.
Contains 1 thioredoxin domain.
Cellular localizationMembrane. Secreted. Cell membrane. Nucleus membrane. Seems to be predominantly targeted to the nuclear and outer plasma membrane.
- Information by UniProt
- DKFZp762A2013 antibody
- Neuroblastoma-derived sulfhydryl oxidase antibody
- QSCN6L1 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in golgi apparatus and vesicles. Recommended concentration of ab121376 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121376 at 1/75 dilution staining QSOX2 in paraffin-embedded Human epididymis tissue by Immunohistochemistry.
All lanes : Anti-QSOX2 antibody (ab121376) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 cell lysate.
Lane 3 : U-251 MG cell lysate.
Lane 4 : Human Plasma
Lane 5 : Human Liver lysate.
Lane 6 : Human Tonsil lysate.
Developed using the ECL technique.
ab121376 has been referenced in 1 publication.
- Kuo YW et al. Identification, characterization and purification of porcine Quiescin Q6-Sulfydryl Oxidase 2 protein. BMC Vet Res 13:205 (2017). PubMed: 28662655