Anti-QSOX2 antibody (ab121376)
Key features and details
- Rabbit polyclonal to QSOX2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-QSOX2 antibody
See all QSOX2 primary antibodies -
Description
Rabbit polyclonal to QSOX2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
internal sequence amino acids 504-633 of Human QSOX2.
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Positive control
- Human epididymis tissue; RT4 and U-251 MG cell lysate; Human Plasma.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. | |
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. Also seems to play a role in regulating the sensitization of neuroblastoma cells for interferon-gamma-induced apoptosis. -
Tissue specificity
Expressed in pancreas, brain, placenta, kidney, heart and fetal tissues. Weakly expressed in lung, liver and skeletal muscles. -
Sequence similarities
Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.
Contains 1 ERV/ALR sulfhydryl oxidase domain.
Contains 1 thioredoxin domain. -
Cellular localization
Membrane. Secreted. Cell membrane. Nucleus membrane. Seems to be predominantly targeted to the nuclear and outer plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 169714 Human
- Omim: 612860 Human
- SwissProt: Q6ZRP7 Human
- Unigene: 657864 Human
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Alternative names
- DKFZp762A2013 antibody
- Neuroblastoma-derived sulfhydryl oxidase antibody
- QSCN6L1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-QSOX2 antibody (ab121376)Immunofluorescent staining of Human cell line U-2 OS shows positivity in golgi apparatus and vesicles. Recommended concentration of ab121376 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-QSOX2 antibody (ab121376)ab121376 at 1/75 dilution staining QSOX2 in paraffin-embedded Human epididymis tissue by Immunohistochemistry. -
Western blot - Anti-QSOX2 antibody (ab121376)All lanes : Anti-QSOX2 antibody (ab121376) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 cell lysate.
Lane 3 : U-251 MG cell lysate.
Lane 4 : Human Plasma
Lane 5 : Human Liver lysate.
Lane 6 : Human Tonsil lysate.
Developed using the ECL technique.
Protocols
Datasheets and documents
References (1)
ab121376 has been referenced in 1 publication.
- Kuo YW et al. Identification, characterization and purification of porcine Quiescin Q6-Sulfydryl Oxidase 2 protein. BMC Vet Res 13:205 (2017). PubMed: 28662655
