Anti-QSOX2 antibody (ab121376)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-QSOX2 antibody
See all QSOX2 primary antibodies -
Description
Rabbit polyclonal to QSOX2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
internal sequence amino acids 504-633 of Human QSOX2.
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Positive control
- Human epididymis tissue; RT4 and U-251 MG cell lysate; Human Plasma.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Function
Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. Also seems to play a role in regulating the sensitization of neuroblastoma cells for interferon-gamma-induced apoptosis. -
Tissue specificity
Expressed in pancreas, brain, placenta, kidney, heart and fetal tissues. Weakly expressed in lung, liver and skeletal muscles. -
Sequence similarities
Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.
Contains 1 ERV/ALR sulfhydryl oxidase domain.
Contains 1 thioredoxin domain. -
Cellular localization
Membrane. Secreted. Cell membrane. Nucleus membrane. Seems to be predominantly targeted to the nuclear and outer plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 169714 Human
- Omim: 612860 Human
- SwissProt: Q6ZRP7 Human
- Unigene: 657864 Human
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Alternative names
- DKFZp762A2013 antibody
- Neuroblastoma-derived sulfhydryl oxidase antibody
- QSCN6L1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-QSOX2 antibody (ab121376)Immunofluorescent staining of Human cell line U-2 OS shows positivity in golgi apparatus and vesicles. Recommended concentration of ab121376 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-QSOX2 antibody (ab121376)ab121376 at 1/75 dilution staining QSOX2 in paraffin-embedded Human epididymis tissue by Immunohistochemistry. -
Western blot - Anti-QSOX2 antibody (ab121376)All lanes : Anti-QSOX2 antibody (ab121376) at 1/250 dilution
Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2 : RT-4 cell lysate.
Lane 3 : U-251 MG cell lysate.
Lane 4 : Human Plasma
Lane 5 : Human Liver lysate.
Lane 6 : Human Tonsil lysate.
Developed using the ECL technique.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Kuo YW et al. Identification, characterization and purification of porcine Quiescin Q6-Sulfydryl Oxidase 2 protein. BMC Vet Res 13:205 (2017). Read more (PubMed: 28662655) »
