Key features and details
- Rabbit polyclonal to QSOX2 - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-QSOX2 antibody - C-terminal
See all QSOX2 primary antibodies
DescriptionRabbit polyclonal to QSOX2 - C-terminal
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
- HeLa, 293T and Jurkat whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/500 - 1/2500. Predicted molecular weight: 78 kDa.|
FunctionCatalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. Also seems to play a role in regulating the sensitization of neuroblastoma cells for interferon-gamma-induced apoptosis.
Tissue specificityExpressed in pancreas, brain, placenta, kidney, heart and fetal tissues. Weakly expressed in lung, liver and skeletal muscles.
Sequence similaritiesBelongs to the quiescin-sulfhydryl oxidase (QSOX) family.
Contains 1 ERV/ALR sulfhydryl oxidase domain.
Contains 1 thioredoxin domain.
Cellular localizationMembrane. Secreted. Cell membrane. Nucleus membrane. Seems to be predominantly targeted to the nuclear and outer plasma membrane.
- Information by UniProt
- DKFZp762A2013 antibody
- Neuroblastoma-derived sulfhydryl oxidase antibody
- QSCN6L1 antibody
All lanes : Anti-QSOX2 antibody - C-terminal (ab191168) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 3 minutes
Lysates prepared using RIPA lysis buffer.
Detection of QSOX2 in immunoprecipitates of HeLa whole cell lysate prepared using RIPA lysis buffer (1 mg for IP, 20% of IP loaded) using ab191168 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab191168 has not yet been referenced specifically in any publications.