Key features and details
- Rabbit polyclonal to QTRTD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-QTRTD1 antibody
DescriptionRabbit polyclonal to QTRTD1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
- WB: RT4 whole cell lysate. IHC-P: Human colon tissue. ICC/IF: A431 cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab247002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 47 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionInteracts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
PathwaytRNA modification; tRNA-queuosine biosynthesis.
Sequence similaritiesBelongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.
Cellular localizationCytoplasm. Mitochondrion. May associate with the mitochondrion outer membrane.
- Information by UniProt
- FLJ12960 antibody
- QTRD1_HUMAN antibody
- qtrtd1 antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for QTRTD1 (green) using ab247002 at 4 μg/ml in ICC/IF.
Paraffin-embedded human colon tissue stained for QTRTD1 using ab247002 at 1/200 dilution in immunohistochemical analysis.
Anti-QTRTD1 antibody (ab247002) at 0.4 µg/ml + RT4 (human urinary bladder cancer cell line) whole cell lysate
Predicted band size: 47 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247002 has not yet been referenced specifically in any publications.