Anti-QTRTD1 antibody (ab247002)

Submit a review Submit a question

Overview

  • Product name

    Anti-QTRTD1 antibody
    See all QTRTD1 primary antibodies

  • Description

    Rabbit polyclonal to QTRTD1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan

  • Immunogen

    Recombinant fragment corresponding to Human QTRTD1 aa 246-324.
    Sequence:

    DKPRLISGVSRPDEVLECIERGVDLFESFFPYQVTERGCALTFSFDYQPN PEETLLQQNGTQEEIKCMDQIKKIETTGC


    Database link: Q9H974

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: RT4 whole cell lysate. IHC-P: Human colon tissue. ICC/IF: A431 cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab247002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function

    Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).

  • Pathway

    tRNA modification; tRNA-queuosine biosynthesis.

  • Sequence similarities

    Belongs to the queuine tRNA-ribosyltransferase family. QTRTD1 subfamily.

  • Cellular localization

    Cytoplasm. Mitochondrion. May associate with the mitochondrion outer membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ12960 antibody
      • QTRD1_HUMAN antibody
      • qtrtd1 antibody
      • queuine tRNA ribosyltransferase domain containing 1 antibody
      • Queuine tRNA-ribosyltransferase domain-containing protein 1 antibody
      • Queuine tRNA-ribosyltransferase subunit qtrtd1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-QTRTD1 antibody (ab247002)
      Immunocytochemistry/ Immunofluorescence - Anti-QTRTD1 antibody (ab247002)

      PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for QTRTD1 (green) using ab247002 at 4 μg/ml in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-QTRTD1 antibody (ab247002)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-QTRTD1 antibody (ab247002)
      Paraffin-embedded human colon tissue stained for QTRTD1 using ab247002 at 1/200 dilution in immunohistochemical analysis.
    • Western blot - Anti-QTRTD1 antibody (ab247002)
      Western blot - Anti-QTRTD1 antibody (ab247002)
      Anti-QTRTD1 antibody (ab247002) at 0.4 µg/ml + RT4 (human urinary bladder cancer cell line) whole cell lysate

      Predicted band size: 47 kDa

    References

    ab247002 has not yet been referenced specifically in any publications.

    Publishing research using ab247002? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab247002.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com