Key features and details
- Antioxidative flavonoid
- CAS Number: 6151-25-3
- Purity: > 97%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Product nameQuercetin, Antioxidative flavonoid
Antioxidative flavonoid. Anticancer, antithrombotic and anti-inflammatory agent. Actions include inhibition of mitochondrial ATPase, phosphodiesterases, PI3-kinase and PIP kinase activity.
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab13847 staining active caspase 3 in A549 cells treated with quercetin (ab120247), by ICC/IF. Increase in active caspase 3 expression correlates with increased concentration of quercetin, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120247 (quercetin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab13847 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab968999) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120247 has been referenced in 2 publications.
- Lin YJ et al. Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells. Phytomedicine 58:152893 (2019). PubMed: 30901663
- Ishida K et al. Inhibition of PI3K suppresses propagation of drug-tolerant cancer cell subpopulations enriched by 5-fluorouracil. Sci Rep 7:2262 (2017). PubMed: 28536445