Recombinant
RabMAb

Recombinant Anti-Quiescin Q6 antibody [EPR21866] (ab235444)

Overview

  • Product name

    Anti-Quiescin Q6 antibody [EPR21866]
    See all Quiescin Q6 primary antibodies
  • Description

    Rabbit monoclonal [EPR21866] to Quiescin Q6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Quiescin Q6 aa 550-650. The exact sequence is proprietary.
    Database link: O00391

  • Positive control

    • WB: BxPC-3, MCF7, MDA-MB-231, PANC-1 and T-47D whole cell lysates; Human breast cancer lysate. IHC-P: Human breast ductal cancer and pancreatic cancer tissue. IP: MDA-MB-231 whole cell lysate. FC: MDA-MB-231 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21866
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 83, 67 kDa (predicted molecular weight: 83 kDa).
Flow Cyt 1/60.
IP 1/30.
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. In fibroblasts, it may have tumor-suppressing capabilities being involved in growth regulation.
  • Tissue specificity

    Expressed in heart, placenta, lung, liver, skeletal muscle, pancreas and very weakly in brain and kidney.
  • Sequence similarities

    Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.
    Contains 1 ERV/ALR sulfhydryl oxidase domain.
    Contains 1 thioredoxin domain.
  • Cellular localization

    Golgi apparatus membrane and Secreted > extracellular space. Found in the extracellular medium of quiescent cells but is not found in proliferating cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ34858 antibody
    • hQSOX antibody
    • Human quiescin (Q6) mRNA, partial cds antibody
    • Q6 antibody
    • QSCN6 antibody
    • QSOX1 antibody
    • QSOX1_HUMAN antibody
    • Quiescin Q6 antibody
    • Quiescin Q6 sulfhydryl oxidase 1 antibody
    • Skin sulfhydryl oxidase antibody
    • Sox antibody
    • Sulfhydryl oxidase 1 antibody
    • Sulfhydryl oxidase 1 precursor antibody
    see all

Images

  • All lanes : Anti-Quiescin Q6 antibody [EPR21866] (ab235444) at 1/1000 dilution

    Lane 1 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : PANC-1 (human pancreatic epithelial cancinoma cell line) whole cell lysate at 10 µg
    Lane 5 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg
    Lane 6 : Human breast cancer lysate at 10 µg

    Secondary
    Lanes 1-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 6 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 83 kDa
    Observed band size: 67,83 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3 & 5: 3 minutes, Lane 4: 37 seconds, Lane 6: 103 seconds.

    Quiescin Q6 has two isoforms due to expression from a long (Quiescin Q6-L) and a short (Quiescin Q6-S) transcript.

  • Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human pancreatic cancer (PMID: 19795908, 21989104) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human breast ductal cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human breast ductal cancer (PMID: 23536962) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Quiescin Q6 with ab235444 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Quiescin Q6 was immunoprecipitated from 0.35 mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab235444 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235444 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: MDA-MB-231 whole cell lysate 10 μg (Input).
    Lane 2: ab235444 IP in MDA-MD-231 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235444 in MDA-MD-231 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    Quiescin Q6 has two isoforms due to expression from a long (Quiescin Q6-L) and a short (Quiescin Q6-S) transcript.

References

ab235444 has not yet been referenced specifically in any publications.

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