Recombinant Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free (ab237600)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21866] to Quiescin Q6 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Quiescin Q6 antibody [EPR21866] - BSA and Azide free
See all Quiescin Q6 primary antibodies -
Description
Rabbit monoclonal [EPR21866] to Quiescin Q6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human pancreatic cancer tissue.
-
General notes
ab237600 is the carrier-free version of ab235444.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21866 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-Quiescin Q6 antibody [EPR21866] (ab235444)
- Alexa Fluor® 488 Anti-Quiescin Q6 antibody [EPR21866] (ab309850)
- Alexa Fluor® 647 Anti-Quiescin Q6 antibody [EPR21866] (ab310216)
- Alexa Fluor® 594 Anti-Quiescin Q6 antibody [EPR21866] (ab310662)
- Alexa Fluor® 555 Anti-Quiescin Q6 antibody [EPR21866] (ab312192)
- Alexa Fluor® 568 Anti-Quiescin Q6 antibody [EPR21866] (ab312680)
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 83, 67 kDa (predicted molecular weight: 83 kDa).
|
|
IP |
Use at an assay dependent concentration.
|
Notes |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 83, 67 kDa (predicted molecular weight: 83 kDa). |
IP
Use at an assay dependent concentration. |
Target
-
Function
Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins. In fibroblasts, it may have tumor-suppressing capabilities being involved in growth regulation. -
Tissue specificity
Expressed in heart, placenta, lung, liver, skeletal muscle, pancreas and very weakly in brain and kidney. -
Sequence similarities
Belongs to the quiescin-sulfhydryl oxidase (QSOX) family.
Contains 1 ERV/ALR sulfhydryl oxidase domain.
Contains 1 thioredoxin domain. -
Cellular localization
Golgi apparatus membrane and Secreted > extracellular space. Found in the extracellular medium of quiescent cells but is not found in proliferating cells. - Information by UniProt
-
Database links
- Entrez Gene: 5768 Human
- Omim: 603120 Human
- SwissProt: O00391 Human
- Unigene: 518374 Human
- Unigene: 719176 Human
-
Alternative names
- FLJ34858 antibody
- hQSOX antibody
- Human quiescin (Q6) mRNA, partial cds antibody
see all
Images
-
Quiescin Q6 was immunoprecipitated from 0.35 mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab235444 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235444 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: MDA-MB-231 whole cell lysate 10 μg (Input).
Lane 2: ab235444 IP in MDA-MD-231 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235444 in MDA-MD-231 whole cell lysate.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.Quiescin Q6 has two isoforms due to expression from a long (Quiescin Q6-L) and a short (Quiescin Q6-S) transcript.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).
-
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling Quiescin Q6 with ab235444 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).
-
Immunohistochemical analysis of paraffin-embedded human breast ductal cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human breast ductal cancer (PMID: 23536962) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).
-
Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling Quiescin Q6 with ab235444 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human pancreatic cancer (PMID: 19795908, 21989104) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235444).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab237600 has not yet been referenced specifically in any publications.