Overview

  • Product name

    Anti-Quinone oxidoreductase antibody
    See all Quinone oxidoreductase primary antibodies
  • Description

    Rabbit polyclonal to Quinone oxidoreductase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Quinone oxidoreductase (internal sequence). The exact sequence is proprietary.
    Database link: Q5HYE7

  • Positive control

    • WB: Jurkat and Raji whole cell lysates; Mouse brain lysate. IHC-P: Human hepatoma tissue. ICC/IF: HepG2 cells.
  • General notes

     This product was previously labelled as CRYZ

     

Properties

Applications

Our Abpromise guarantee covers the use of ab229535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 35 kDa.

Target

  • Function

    Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.
  • Tissue specificity

    Only very low amounts in the lens.
  • Sequence similarities

    Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Crystallin zeta (quinone reductase) antibody
    • Crystallin zeta antibody
    • Cryz antibody
    • DKFZp779E0834 antibody
    • FLJ41475 antibody
    • NADPH:quinone reductase antibody
    • QOR_HUMAN antibody
    • Quinone oxidoreductase antibody
    • Zeta crystallin antibody
    • Zeta-crystallin antibody
    see all

Images

  • Anti-Quinone oxidoreductase antibody (ab229535) at 1/500 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-Quinone oxidoreductase antibody (ab229535) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded human hepatoma tissue stained for Quinone oxidoreductase using ab229535 at 1/500 dilution in immunohistochemical analysis.

  • Ice cold methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Quinone oxidoreductase (green) using ab229535 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229535 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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