Key features and details
- Rabbit polyclonal to Quinone oxidoreductase
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Quinone oxidoreductase antibody
See all Quinone oxidoreductase primary antibodies
DescriptionRabbit polyclonal to Quinone oxidoreductase
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human Quinone oxidoreductase (internal sequence). The exact sequence is proprietary.
Database link: Q5HYE7
- WB: Jurkat and Raji whole cell lysates; Mouse brain lysate. IHC-P: Human hepatoma tissue. ICC/IF: HepG2 cells.
This product was previously labelled as CRYZ
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 35 kDa.|
FunctionDoes not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.
Tissue specificityOnly very low amounts in the lens.
Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
- Information by UniProt
- Crystallin zeta (quinone reductase) antibody
- Crystallin zeta antibody
- Cryz antibody
Anti-Quinone oxidoreductase antibody (ab229535) at 1/500 dilution + Mouse brain lysate at 50 µg
Predicted band size: 35 kDa
10% SDS-PAGE gel.
All lanes : Anti-Quinone oxidoreductase antibody (ab229535) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
10% SDS-PAGE gel.
Paraffin-embedded human hepatoma tissue stained for Quinone oxidoreductase using ab229535 at 1/500 dilution in immunohistochemical analysis.
Ice cold methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Quinone oxidoreductase (green) using ab229535 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229535 has not yet been referenced specifically in any publications.