Recombinant
RabMAb

Recombinant Anti-R Cadherin antibody [EPR4836] (ab109242)

Overview

  • Product name

    Anti-R Cadherin antibody [EPR4836]
    See all R Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EPR4836] to R Cadherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human R Cadherin aa 800-900. The exact sequence is proprietary.

  • Positive control

    • HepG2 whole cell lysate (ab7900), fetal brain lysate, SH-SY5Y cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 100 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    Images

    • All lanes : Anti-R Cadherin antibody [EPR4836] (ab109242) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg/ml per lane.

      Predicted band size: 100 kDa

    References

    ab109242 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for the protocol and sample details.

    The 100kDa band may be cadherin. The size of the protein, without any post-translational modification such as glycosylation, is expected to be 100 kDa, according to the UniProt database entry for the mouse CADH4. Are these osteosarcoma cell lines known to glycosylate CADH4? Here is a link to the entry. The amino acid sequence and size are listed far down the page:

    http://www.uniprot.org/uniprot/P39038

    The 80 kDa band may represent a fragment. Can you please confirm that you collected your samples with protease inhibitors? Have you tried staining blots of these samples with any other antibodies?

    The recommended dilution is a range from 1/10,000 to 1/50,000. The antibody has very high affinity for R cadherin, so I recommend stripping a blot and re-staining with a more dilute solution of the antibody, for instance 1/25,000. This should reduce the amount of background staining.

    Please let me know if you have any questions or concerns.

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    Answer

    Thank you for bringing this to our attention. Can you please provide some details of your samples and protocol? For instance, what is the species and tissue type or cell type, how were the samples prepared, how much sample did you load per lane, what did you use for a blocking buffer, and what was the dilution or concentration of the antibody? Are you sure the secondary antibody is not the problem?

    Can you please also send the order details? The name of the institution and the approximate date of the order will be sufficient.

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