Biotin Anti-R Phycoerythrin/rpeA antibody (ab34730)
Key features and details
- Biotin Goat polyclonal to R Phycoerythrin/rpeA
- Suitable for: Dot blot, WB, ELISA
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-R Phycoerythrin/rpeA antibody
See all R Phycoerythrin/rpeA primary antibodies -
Description
Biotin Goat polyclonal to R Phycoerythrin/rpeA -
Host species
Goat -
Conjugation
Biotin -
Specificity
This antibody will cross react with B Phycoerythrin. Reactivity with other phycobiliproteins is unknown. -
Tested applications
Suitable for: Dot blot, WB, ELISAmore details -
Species reactivity
Reacts with: Red Algae -
Immunogen
Full length protein corresponding to R Phycoerythrin/rpeA.
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General notes
10-20 Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) molecules per goat IgG molecule.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride
IgG and Protease free -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
Use at an assay dependent dilution.
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WB |
1/1000 - 1/3000. Detects a band of approximately 240 kDa.
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ELISA |
1/8000 - 1/32000.
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Notes |
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Dot blot
Use at an assay dependent dilution. |
WB
1/1000 - 1/3000. Detects a band of approximately 240 kDa. |
ELISA
1/8000 - 1/32000. |
Target
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Relevance
Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents. -
Cellular localization
Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome. -
Alternative names
- R phycoerythrin alpha subunit antibody
- R phycoerythrin beta subunit antibody
- rpeA antibody
- rpeB antibody
Datasheets and documents
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Datasheet download
References (0)
ab34730 has not yet been referenced specifically in any publications.