Overview

  • Product name

    Anti-R Phycoerythrin/rpeA antibody
    See all R Phycoerythrin/rpeA primary antibodies
  • Description

    Goat polyclonal to R Phycoerythrin/rpeA
  • Host species

    Goat
  • Specificity

    This antibody cross reacts with B Phycoerythrin.
  • Tested applications

    Suitable for: ELISA, WB, IP, Immunodiffusion, Conjugationmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length native protein (purified) corresponding to Sea anenome R Phycoerythrin/rpeA. Purified from the seaweed Gracilaria.

  • General notes

     This product was previously labelled as R Phycoerythrin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was purified from monospecific antiserum by immunoaffinity chromatography using a R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IP
Immunodiffusion
Conjugation
  • Application notes
    Conjugation to magnetic particles (beads): Use at an assay dependent dilution.
    ELISA: 1/20,000 - 1/100,000.
    ID: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: 1/2,000 - 1/10,000. Predicted molecular weight: 240 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
    • Cellular localization

      Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
    • Alternative names

      • R phycoerythrin alpha subunit antibody
      • R phycoerythrin beta subunit antibody
      • rpeA antibody
      • rpeB antibody

    References

    ab34721 has not yet been referenced specifically in any publications.

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