Anti-R Phycoerythrin/rpeA antibody (Alkaline Phosphatase) (ab34729)

Overview

  • Product name

    Anti-R Phycoerythrin/rpeA antibody (Alkaline Phosphatase)
    See all R Phycoerythrin/rpeA primary antibodies
  • Description

    Goat polyclonal to R Phycoerythrin/rpeA (Alkaline Phosphatase)
  • Host species

    Goat
  • Conjugation

    Alkaline Phosphatase
  • Tested applications

    Suitable for: Dot blot, ELISA, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to Goat R Phycoerythrin/rpeA.

  • General notes

    Alkaline Phosphatase (from Calf Intestine, Molecular Weight 140 kDa). Conjugation Reference: Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.

     This product was previously labelled as R Phycoerythrin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.79% Tris HCl, 0.0095% Magnesium chloride, 0.00136% Zinc chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified by immunoaffinity chromatography using a R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab34729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
ELISA 1/1000 - 1/5000.
WB Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.

Target

  • Relevance

    Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
  • Cellular localization

    Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
  • Alternative names

    • R phycoerythrin alpha subunit antibody
    • R phycoerythrin beta subunit antibody
    • rpeA antibody
    • rpeB antibody

References

ab34729 has not yet been referenced specifically in any publications.

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