Overview

  • Product name

    Anti-R Phycoerythrin/rpeA antibody (FITC)
    See all R Phycoerythrin/rpeA primary antibodies
  • Description

    Goat polyclonal to R Phycoerythrin/rpeA (FITC)
  • Host species

    Goat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    This antibody cross reacts with B Phycoerythrin.
  • Tested applications

    Suitable for: Immunomicroscopy, Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length native protein (purified) corresponding to R Phycoerythrin/rpeA. Highly purified R Phycoerythrin/rpeA from the seaweed Gracilaria.

  • General notes

     This product was previously labelled as R Phycoerythrin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using a R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.

The antibody is also thought to be suitable for other antibody based fluorescent assays.

 

Flow Cyt Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
  • Cellular localization

    Plastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
  • Alternative names

    • R phycoerythrin alpha subunit antibody
    • R phycoerythrin beta subunit antibody
    • rpeA antibody
    • rpeB antibody

References

This product has been referenced in:

  • Liu WH  et al. CD63 inhibits the cell migration and invasion ability of tongue squamous cell carcinoma. Oncol Lett 15:9033-9042 (2018). Read more (PubMed: 29844819) »
See 1 Publication for this product

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