Key features and details
- Goat polyclonal to R Phycoerythrin/rpeA (HRP)
- Suitable for: WB, Dot blot, ELISA, Electron Microscopy
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Product nameAnti-R Phycoerythrin/rpeA antibody (HRP)
See all R Phycoerythrin/rpeA primary antibodies
DescriptionGoat polyclonal to R Phycoerythrin/rpeA (HRP)
SpecificityThis antibody cross reacts with B Phycoerythrin.
Tested applicationsSuitable for: WB, Dot blot, ELISA, Electron Microscopymore details
Species reactivityReacts with: Species independent
Full length protein corresponding to R Phycoerythrin/rpeA. Corresponds to the seaweed Gracilaria
This product was previously labelled as R Phycoerythrin
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis antibody was prepared from monospecific antiserum by immunoaffinity chromatography using a R Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab34727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/2,000 - 1/10,000.
WB: 1/500 - 1/2,000. Predicted molecular weight: 240 kDa.
Not yet tested in other applications but thought to be suitable for other peroxidase-antibody based enzymatic assays.
Optimal dilutions/concentrations should be determined by the end user.
RelevancePhycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
Cellular localizationPlastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
- R phycoerythrin alpha subunit antibody
- R phycoerythrin beta subunit antibody
- rpeA antibody
- rpeB antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab34727 has not yet been referenced specifically in any publications.