Key features and details
- Goat polyclonal to R Phycoerythrin/rpeA (Rhodamine)
- Suitable for: Immunomicroscopy, Flow Cyt, WB
- Reacts with: Species independent
- Conjugation: Rhodamine. Ex: 550nm, Em: 570nm
- Isotype: IgG
Product nameAnti-R Phycoerythrin/rpeA antibody (Rhodamine)
See all R Phycoerythrin/rpeA primary antibodies
DescriptionGoat polyclonal to R Phycoerythrin/rpeA (Rhodamine)
ConjugationRhodamine. Ex: 550nm, Em: 570nm
This antibody recognizes R Phycoerythrin/rpeA conjugated IgG. It will also cross react with B Phycoerythrin.
Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, WBmore details
Species reactivityReacts with: Species independent
Full length native protein (purified) corresponding to R Phycoerythrin/rpeA.
Label: Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons). Absorption Wavelength: 550 nm. Emission Wavelength: 570 nm. Fluorochrome/Protein Ratio: 4.0 moles TRITC per mole of Goat IgG.
This product was previously labelled as R Phycoerythrin
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from monospecific antiserun by immunoaffinity chromatography using a R Phycoerythrin/rpeA coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities.
Our Abpromise guarantee covers the use of ab34717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Immunomicroscopy||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 240 kDa.|
RelevancePhycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.
Cellular localizationPlastid; chloroplast; chloroplast thylakoid lumen. Periphery of the rods of the phycobilisome.
- R phycoerythrin alpha subunit antibody
- R phycoerythrin beta subunit antibody
- rpeA antibody
- rpeB antibody
ab34717 has been referenced in 1 publication.
- Fujita T et al. Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep 3:3171 (2013). PubMed: 24201379