Product nameAnti-R2D2 antibody
DescriptionRabbit polyclonal to R2D2
SpecificityThe antibody specifically recognises the immunising peptide in a blocking western blot. All four observed bands are blocked, showing that they are specific. There is also low background and high titre in ELISA. Please let us know if you can explain these bands.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Drosophila melanogaster
Synthetic peptide corresponding to Drosophila melanogaster R2D2 aa 100-200 (intracellular) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Drosophila lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab27650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).|
RelevanceThe RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme consists of DCR-2 and R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4.
- CG7138 antibody
- CG7138 PA antibody
- LD06392p antibody
All lanes : Anti-R2D2 antibody (ab27650) at 1 µg/ml
Lane 1 : Drosophila lysate
Lane 2 : Drosophila lysate with Human R2D2 peptide (ab17670) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa (possible cross reactivity), 55 kDa (possible cross reactivity), 85 kDa (possible cross reactivity)
ab27650 specifically recognises a band at 37kDa corresponding to R2D2, which is blocked using the immunising peptide (ab17670).
ab27650 has not yet been referenced specifically in any publications.