Overview

  • Product name

    Anti-R2D2 antibody
  • Description

    Rabbit polyclonal to R2D2
  • Host species

    Rabbit
  • Specificity

    The antibody specifically recognises the immunising peptide in a blocking western blot. All four observed bands are blocked, showing that they are specific. There is also low background and high titre in ELISA. Please let us know if you can explain these bands.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Drosophila melanogaster R2D2 aa 100-200 (intracellular) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab17670)

  • Positive control

    • This antibody gave a positive signal in Drosophila lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).

Target

  • Relevance

    The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme consists of DCR-2 and R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4.
  • Cellular localization

    Nuclear
  • Database links

    • Alternative names

      • CG7138 antibody
      • CG7138 PA antibody
      • LD06392p antibody

    Images

    • All lanes : Anti-R2D2 antibody (ab27650) at 1 µg/ml

      Lane 1 : Drosophila lysate
      Lane 2 : Drosophila lysate with Human R2D2 peptide (ab17670) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 35 kDa
      Observed band size: 37 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 39 kDa (possible cross reactivity), 55 kDa (possible cross reactivity), 85 kDa (possible cross reactivity)



      ab27650 specifically recognises a band at 37kDa corresponding to R2D2, which is blocked using the immunising peptide (ab17670).

    References

    ab27650 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab27650.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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