Overview

  • Product name

    Anti-Rab-6 antibody
  • Description

    Rabbit polyclonal to Rab-6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human Rab-6 aa 1-178.

  • Positive control

    • H1299 whole cell lysate HeLa cells MDA-MB-468 xenograft tissue
  • General notes

    We have not tested the cross reactivity to RAB6B. ab95954 is raised against a full length RAB6A recombinant protein and may cross-react with RAB6B.

    Protein previously labeled as RAB6A.

Properties

Applications

Our Abpromise guarantee covers the use of ab95954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

Images

  • Anti-Rab-6 antibody (ab95954) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 24 kDa

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab95954 at 1:200 dilution (1) and merged with DNA probe (2).
  • ab95954, at a 1:500 dilution, staining human Rab-6 in MDA-MB 468 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

References

This product has been referenced in:

  • Abdullah MI  et al. Inhibition of the mevalonate pathway augments the activity of pitavastatin against ovarian cancer cells. Sci Rep 7:8090 (2017). Read more (PubMed: 28808351) »
  • Hou X  et al. Rab6a is a novel regulator of meiotic apparatus and maturational progression in mouse oocytes. Sci Rep 6:22209 (2016). Read more (PubMed: 26915694) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mice Hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
35 µg
Specification
Mice Hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 20 2019

Application
Western blot
Sample
Rat Cell lysate - other (Rat hepatocytes plasma membrane fraction)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
35 µg
Specification
Rat hepatocytes plasma membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Oct 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
No
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Oct 28 2014

Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP prostate cancer cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
LNCaP prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Nov 13 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Panc-1)
Permeabilization
No
Specification
Panc-1
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Dec 03 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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