Key features and details
- Rabbit polyclonal to RAB
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAB antibody
See all RAB primary antibodies
DescriptionRabbit polyclonal to RAB
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to Human RAB aa 512-562.
Database link: NP_004495.2
- HeLa or 293T cell lysate.
This product was previously labelled as AGFG1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 58 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRequired for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.
Tissue specificityUbiquitously expressed.
Sequence similaritiesContains 1 Arf-GAP domain.
DomainContains FG repeats. The FG repeat region is required for acting as a cofactor of HIV-1 Rev.
Cellular localizationNucleus. Cytoplasmic vesicle.
- Information by UniProt
- AGFG1 antibody
- AGFG1_HUMAN antibody
- Arf-GAP domain and FG repeats-containing protein 1 antibody
All lanes : Anti-RAB antibody (ab86349) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 58 kDa
Exposure time: 10 seconds
Detection of RAB by Western Blot of Immunprecipitate.
ab86349 at 1µg/ml staining RAB in HeLa whole cell lysate immunoprecipitated using ab86349 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
ab86349 has been referenced in 1 publication.
- Kubota Y et al. WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins. PLoS One 12:e0180714 (2017). Human . PubMed: 28686627