Overview

  • Product name

  • Description

    Rabbit polyclonal to RAB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human RAB aa 512-562.
    Database link: NP_004495.2

  • Positive control

    • HeLa or 293T cell lysate.
  • General notes

     This product was previously labelled as AGFG1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab86349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Required for vesicle docking or fusion during acrosome biogenesis (By similarity). May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element-containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Contains 1 Arf-GAP domain.
  • Domain

    Contains FG repeats. The FG repeat region is required for acting as a cofactor of HIV-1 Rev.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Nucleus. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGFG1 antibody
    • AGFG1_HUMAN antibody
    • Arf-GAP domain and FG repeats-containing protein 1 antibody
    • ArfGAP with FG repeats 1 antibody
    • AU045498 antibody
    • C130049H11Rik antibody
    • C85612 antibody
    • D730048C23Rik antibody
    • DKFZp686I15205 antibody
    • HIV 1 Rev binding protein antibody
    • HIV-1 Rev-binding protein antibody
    • HRB antibody
    • MGC116375 antibody
    • MGC116938 antibody
    • MGC116940 antibody
    • Nucleoporin like protein RIP antibody
    • Nucleoporin-like protein RIP antibody
    • RAB antibody
    • Rev interacting protein antibody
    • Rev-interacting protein antibody
    • Rev/Rex activation domain-binding protein antibody
    • RIP antibody
    see all

Images

  • All lanes : Anti-RAB antibody (ab86349) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 58 kDa


    Exposure time: 10 seconds
  • Detection of RAB by Western Blot of Immunprecipitate.
    ab86349 at 1µg/ml staining RAB in HeLa whole cell lysate immunoprecipitated using ab86349 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

References

This product has been referenced in:

  • Kubota Y  et al. WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins. PLoS One 12:e0180714 (2017). Human . Read more (PubMed: 28686627) »
See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

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Verified customer

Submitted Jul 22 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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