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What is the homology of the immunogen with drosophila?
Abcam community
Verified customer
Asked on Sep 26 2011
Thank you for your inquiry. Based on the BLAST results, the immunogen sequence used to generate ab104859 is only 69% homologous with drosophila. This is a bit low, so cross-reactivity with drosophila would probably not be expected with this antibody. Our other RAB10 antibodies, ab110517 and ab113947, have 90% and ~60% homologies, respectively, with drosophila. Therefore, I would recommend ab110517 since this has the highest homology, but again since it hasn't been tested in drosophila we wouldn't be able to guarantee cross-reactivity. To our knowledge, ab110517 has not been tested in drosophila. Therefore, I can offer a discount off a future purchase if you buy ab110517 now, test it in drosophila and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab110517 in drosphila. I will then send a discount code. This code must be issued before purchasing ab110517 so please wait for my reply before ordering. 2. Purchase ab110517 either by phone, fax, or online (www.abcam.com). 3. Test it in drosophila. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab110517 turns out to be unsuitable for drosophila, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.
Answered on Sep 26 2011