Recombinant
RabMAb

Recombinant Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)

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Overview

  • Product name

    Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5]
    See all RAB10 primary antibodies

  • Description

    Rabbit monoclonal [MJF-R21-22-5] to RAB10 (phospho T73)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Dot blot, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human RAB10 aa 50-150 (phospho T73). The exact sequence is proprietary.
    Database link: P61026

  • Positive control

    • WB: HEK293 overexpressing HA-tagged Rab10, with MLi2 treatment whole cell lysate. ICC/IF: MLi-2 treated MEF cells. Dot Blot: Rab10 (phospho T73) peptide.

  • General notes

    This antibody was developed with support from The Michael J. Fox Foundation.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab241060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
Dot blot 1/1000.
ICC/IF 1/100.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. According to PubMed:23263280, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.

    • Sequence similarities

      Belongs to the small GTPase superfamily. Rab family.

    • Cellular localization

      Cytoplasmic vesicle membrane. Golgi apparatus membrane. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, phagosome membrane. Cell projection, cilium. Endoplasmic reticulum membrane. Associates with SLC2A4/GLUT4 storage vesicles (PubMed:22908308). Localizes to the base of the cilium (PubMed:20576682). Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (PubMed:23263280).
    • Information by UniProt

    • Database links

      • Alternative names

        • GTP binding protein RAB10 antibody
        • Rab10 antibody
        • RAB10 member RAS oncogene family antibody
        • RAB10_HUMAN antibody
        • Ras related GTP binding protein antibody
        • Ras related GTP binding protein RAB10 antibody
        • Ras-related protein Rab-10 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)
        Immunocytochemistry/ Immunofluorescence - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Saponin permeabilized MEF (mouse embryonic fibroblast) generated from wild-type or mutant LRRK2[R1441C] embryos without and with (+) MLi-2 treatment (100nM for 60 min) cells labeling RAB10 (phospho T73) with ab241060 at 0.5 μg/mL, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)  secondary antibody at 1/1000 dilution (red). Nuclear counterstain is DAPI.

        The image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

      • Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)
        Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)
        All lanes : Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060) at 1/500 dilution

        Lane 1 : HEK293 (human embryonic kidney epithelial cells) overexpressing HA-tagged Rab3, whole cell lysate
        Lane 2 : HEK293 overexpressing HA-tagged Rab3, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
        Lane 3 : HEK293 overexpressing HA-tagged Rab5, whole cell lysate
        Lane 4 : HEK293 overexpressing HA-tagged Rab5, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
        Lane 5 : HEK293 overexpressing HA-tagged Rab8, whole cell lysate
        Lane 6 : HEK293 overexpressing HA-tagged Rab8, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
        Lane 7 : HEK293 overexpressing HA-tagged Rab10, whole cell lysate
        Lane 8 : HEK293 overexpressing HA-tagged Rab10, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
        Lane 9 : HEK293 overexpressing HA-tagged Rab12, whole cell lysate
        Lane 10 : HEK293 overexpressing HA-tagged Rab12, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
        Lane 11 : HEK293 overexpressing HA-tagged Rab29, whole cell lysate
        Lane 12 : HEK293 overexpressing HA-tagged Rab29, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
        Lane 13 : HEK293 overexpressing HA-tagged Rab35, whole cell lysate
        Lane 14 : HEK293 overexpressing HA-tagged Rab35, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate
        Lane 15 : HEK293 overexpressing HA-tagged Rab43, whole cell lysate
        Lane 16 : HEK293 overexpressing HA-tagged Rab43, with (+) MLi2 treatment (100nM for 60 min), whole cell lysate

        Lysates/proteins at 3 µg per lane.

        Secondary
        All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution

        Predicted band size: 22 kDa



        Blocking buffer: 5% NFDM/TBST.

        Dilution buffer: 5% BSA/TBST.

        MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).

        The blot was scanned with LI-COR Odyssey CLx.

        This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

      • Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)
        Dot Blot - Anti-RAB10 (phospho T73) antibody [MJF-R21-22-5] (ab241060)

        Dot Blot - Anti-RAB10 antibody [MJF-R21-22-5] (ab241060) at a 1/1000 dilution.

        Lane 1: Rab10 (phospho T73) peptide

        Lane 2: Rab10 non-phospho peptide

        Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at a 1/100000 dilution.

        Blocking/Dilution buffer: 5% NFDM/TBST.

        Exposure time: 180 seconds.

      References

      ab241060 has not yet been referenced specifically in any publications.

      Publishing research using ab241060? Please let us know so that we can cite the reference in this datasheet.

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