Anti-Rab11 antibody (ab3612)

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This antibody detects both RAB11A and Rab11B.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply. I would recommend keeping the blocking to one hour and incubating the primary antibody overnight at 4 degrees. I would suggest doing this at 1/300 for ab74906 and at 1/1000 for ab3612. Please let me know if this does not improve your results.

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If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

As shown on the product datasheet, the Rabbit polyclonal anti-Rab11 ab3612 (https://www.abcam.com/ab3612) is guaranteed to work in ICC/IF, Western Blot and on Human, Dog, Non Human Primates samples.
The other anti-Rab11 we have in our catalogue is a Rat monoclonal, reference ab95375 (https://www.abcam.com/ab95375). Like ab3612 it has been tested in ICC/IF and Western Blot but only on Mouse samples.

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Thank you for the further information. It is difficult to select alternatives that are the most 'specific' as we have not tested these antibodies side by side in the same sample application or species. Reviewing the datasheets I think that ab74906 anti-Rab7 antibody and ab3612 anti-Rabb11 antibody may be preferable but please do check the datasheets to confirm whether you agree. Please bear in mind that ab74906 (rabbit polyclonal to Rab7) is not tested in ICC, and as it shows a weak band in WB the staining intensity may be lower than other antibodies. In addition, ab3612 (rabbit polyclonal to Rab11) is only predicted to work in mouse samples, and may not give positive results. As the Rab7 and Rab11 antibodies will be used in previously untested species/applications, would it be possible to submit an Abreview with your results? It doesn't matter if it is positive or negative, but the information will be really useful for other researchers that may be interested in using these products in a similar manner. I will be able to provide ab78970 and ab87802. Could you also submit Abreview information for these products too? Any other information you be able to share via our Abreview system would be extremely useful. Looking forward to your reply.

Thank you for contacting us. Yes, a suggested starting dilution for the primary antibody would be 1/200. And I would suggest keeping the secondary antibody dilution constant while titrating the primary antibody. Once you know the optimal dilution for the primary you could try a higher and a lower dilution of the secondary to see if any other dilution might give even better results. But it is often advisable to change only one parameter the time. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.