Overview

  • Product name

    Anti-Rab11 antibody [KT80]
    See all Rab11 primary antibodies
  • Description

    Rat monoclonal [KT80] to Rab11
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Fusion protein corresponding to Mouse Rab11.
    Database link: P46638

Properties

Applications

Our Abpromise guarantee covers the use of ab95375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 24 kDa.
ICC/IF 1/100.

Target

  • Function

    GTPase that modulates endosomal trafficking. Acts as a major regulator of membrane delivery during cytokinesis.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTP binding protein YPT3 antibody
    • GTP-binding protein ypt3 antibody
    • H-YPT3 antibody
    • RAB 11A antibody
    • RAB11B antibody
    • RAB11B member RAS oncogene family antibody
    • RAB11B, member of RAS oncogene family antibody
    • Ras related protein Rab 11B antibody
    • RAS-associated protein RAB11B antibody
    • Ras-related protein Rab-11B antibody
    • RB11B_HUMAN antibody
    • YPT3 antibody
    see all

References

This product has been referenced in:

  • Del Cid-Pellitero E  et al. Homeostatic Changes in GABA and Glutamate Receptors on Excitatory Cortical Neurons during Sleep Deprivation and Recovery. Front Syst Neurosci 11:17 (2017). Read more (PubMed: 28408870) »
  • Tjondrokoesoemo A  et al. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet 25:1192-202 (2016). Read more (PubMed: 26744329) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (liver, PLC5 cell-line)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Treatment
starvation for 12 hours (medium without FBS)
Specification
liver, PLC5 cell-line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Miss. Yu-Shan Chen

Verified customer

Submitted Aug 24 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 6 · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Aug 12 2016

Answer

Thank you for your enquiry.

I am sorry but we do not have blocking peptides available for ab50533 or ab95375.

Please feel free to contact me again if you have any further questions.

Read More

Question
Answer

Thank you for the further information. It is difficult to select alternatives that are the most 'specific' as we have not tested these antibodies side by side in the same sample application or species. Reviewing the datasheets I think that ab74906 anti-Rab7 antibody and ab3612 anti-Rabb11 antibody may be preferable but please do check the datasheets to confirm whether you agree. Please bear in mind that ab74906 (rabbit polyclonal to Rab7) is not tested in ICC, and as it shows a weak band in WB the staining intensity may be lower than other antibodies. In addition, ab3612 (rabbit polyclonal to Rab11) is only predicted to work in mouse samples, and may not give positive results. As the Rab7 and Rab11 antibodies will be used in previously untested species/applications, would it be possible to submit an Abreview with your results? It doesn't matter if it is positive or negative, but the information will be really useful for other researchers that may be interested in using these products in a similar manner. I will be able to provide ab78970 and ab87802. Could you also submit Abreview information for these products too? Any other information you be able to share via our Abreview system would be extremely useful. Looking forward to your reply.

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