Overview

  • Product name

    Anti-RAB11-FIP2 antibody [EPR12294-85]
    See all RAB11-FIP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12294-85] to RAB11-FIP2
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human RAB11-FIP2 aa 250-450. The exact sequence is proprietary.
    Database link: Q7L804

  • Positive control

    • 293 and HeLa whole cell lysate (ab150035); Human brain tissue; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12294-85
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/500.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
See IHC antigen retrieval protocols.
For unpurified use at 1/250. 

ICC/IF 1/100.

Target

  • Function

    A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 FIP-RBD domain.
  • Post-translational
    modifications

    Phosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity.
  • Cellular localization

    Cell membrane. Recycling endosome membrane. Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0941 antibody
    • NRip11 antibody
    • RAB11 family interacting protein 2 (class I) antibody
    • Rab11 family-interacting protein 2 antibody
    • RAB11 FIP2 long isoform antibody
    • Rab11-FIP2 antibody
    • RAB11FIP2 antibody
    • RFIP2 antibody
    • RFIP2_HUMAN antibody
    see all

Images

  • All lanes : Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504) at 1/10000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling RAB11-FIP2 with Purified ab180504 at 1:500 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence analysis of HEK-293 (human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1:100 (5.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling RAB11-FIP2 with purified ab180504 at 1:50 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • All lanes : Anti-RAB11-FIP2 antibody [EPR12294-85] (ab180504) at 1/10000 dilution (unpurified)

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa

  • Immunohistochemical analysis of paraffin-embedded, Human brain tissue labeling RAB11-FIP2 with unpurified 
    ab180504 at a 1/250 dilution. Counter stained with hematoxylin.

  • Immunofluorescence analysis of, paraformaldehyde-fixed, 293 cells labeling RAB11-FIP2 with unpurified 
    ab180504 at a 1/100 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/250. In blue DAPI staining.

References

This product has been referenced in:

  • Skjesol A  et al. The TLR4 adaptor TRAM controls the phagocytosis of Gram-negative bacteria by interacting with the Rab11-family interacting protein 2. PLoS Pathog 15:e1007684 (2019). Read more (PubMed: 30883606) »
See 1 Publication for this product

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