Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12294] to RAB11-FIP2
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Human
Product nameAnti-RAB11-FIP2 antibody [EPR12294]
See all RAB11-FIP2 primary antibodies
DescriptionRabbit monoclonal [EPR12294] to RAB11-FIP2
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human RAB11-FIP2. The exact sequence is proprietary.
Database link: Q7L804
- 293T and HeLa cell lysates; permeabilized HeLa cells; Immunoprecipitation pellet from 293T cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 58 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionA Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity.
Sequence similaritiesContains 1 C2 domain.
Contains 1 FIP-RBD domain.
modificationsPhosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity.
Cellular localizationCell membrane. Recycling endosome membrane. Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane.
- Information by UniProt
- KIAA0941 antibody
- NRip11 antibody
- RAB11 family interacting protein 2 (class I) antibody
All lanes : Anti-RAB11-FIP2 antibody [EPR12294] (ab174313) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 58 kDa
Flow cytometric analysis of permeabilized HeLa cells using ab174313 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Western blot analysis of immunoprecipitation pellet from 293T cell lysate (lane 1) or 1XPBS (negative control) (lane 2) using ab174313 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab174313 has been referenced in 1 publication.
- He K et al. Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules. Nat Commun 9:3310 (2018). PubMed: 30120249