Overview

  • Product name

    Anti-RAB11-FIP2 antibody [EPR12294]
    See all RAB11-FIP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12294] to RAB11-FIP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RAB11-FIP2. The exact sequence is proprietary.
    Database link: Q7L804

  • Positive control

    • 293T and HeLa cell lysates; permeabilized HeLa cells; Immunoprecipitation pellet from 293T cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12294
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity.
    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 FIP-RBD domain.
    • Post-translational
      modifications

      Phosphorylation at Ser-227 by MARK2 regulates epithelial cell polarity.
    • Cellular localization

      Cell membrane. Recycling endosome membrane. Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • KIAA0941 antibody
      • NRip11 antibody
      • RAB11 family interacting protein 2 (class I) antibody
      • Rab11 family-interacting protein 2 antibody
      • RAB11 FIP2 long isoform antibody
      • Rab11-FIP2 antibody
      • RAB11FIP2 antibody
      • RFIP2 antibody
      • RFIP2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-RAB11-FIP2 antibody [EPR12294] (ab174313) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 58 kDa

    • Flow cytometric analysis of permeabilized HeLa cells using ab174313 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Western blot analysis of immunoprecipitation pellet from 293T cell lysate (lane 1) or 1XPBS (negative control) (lane 2) using ab174313 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:

    • He K  et al. Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules. Nat Commun 9:3310 (2018). Read more (PubMed: 30120249) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    HEK cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Aug 15 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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