Overview

  • Product name

    Anti-Rab11A antibody [EPR7587(B)]
    See all Rab11A primary antibodies
  • Description

    Rabbit monoclonal [EPR7587(B)] to Rab11A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Rab11A aa 150 to the C-terminus.
    Database link: P62491

  • Positive control

    • WB: PC12, HeLa and A549 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
IP 1/10 - 1/100.

Target

  • Function

    Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane. Recycling endosome membrane. Cleavage furrow. Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC1490 antibody
    • Rab 11 antibody
    • Rab 11A antibody
    • RAB 11A, member oncogene family antibody
    • Rab-11 antibody
    • RAB11 A antibody
    • RAB11 antibody
    • Rab11a antibody
    • RAB11A member RAS oncogene family antibody
    • Ras related protein Rab 11A antibody
    • Ras related protein Rab11A antibody
    • Ras-related protein Rab-11A antibody
    • RB11A_HUMAN antibody
    • YL 8 antibody
    • YL8 antibody
    see all

Images

  • All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified)

    Lane 1 : Rat brain lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified)

    Lane 1 : Mouse brain cell lysate
    Lane 2 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa

  • Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified) + A549 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (unpurified)

    Lane 1 : PC12 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 24 kDa

  • ab128913 (purified) at 1/20 immunoprecipitating Rab11A in A549 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

References

This product has been referenced in:

  • Li H  et al. Coronary Serum Exosomes Derived from Patients with Myocardial Ischemia Regulate Angiogenesis through the miR-939-mediated Nitric Oxide Signaling Pathway. Theranostics 8:2079-2093 (2018). Read more (PubMed: 29721064) »
  • Puri C  et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell 45:114-131.e8 (2018). WB, ICC/IF . Read more (PubMed: 29634932) »
See all 6 Publications for this product

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