Recombinant
RabMAb

Recombinant Anti-Rab11A antibody [EPR7587(B)] - BSA and Azide free (ab240036)

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Overview

  • Product name

    Anti-Rab11A antibody [EPR7587(B)] - BSA and Azide free
    See all Rab11A primary antibodies

  • Description

    Rabbit monoclonal [EPR7587(B)] to Rab11A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Rab11A aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P62491

  • General notes

    Ab240036 is the carrier-free version of ab128913. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240036 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.

Target

  • Function

    Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.

  • Cellular localization

    Cell membrane. Recycling endosome membrane. Cleavage furrow. Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • MGC1490 antibody
    • Rab 11 antibody
    • Rab 11A antibody
    • RAB 11A, member oncogene family antibody
    • Rab-11 antibody
    • RAB11 A antibody
    • RAB11 antibody
    • Rab11a antibody
    • RAB11A member RAS oncogene family antibody
    • Ras related protein Rab 11A antibody
    • Ras related protein Rab11A antibody
    • Ras-related protein Rab-11A antibody
    • RB11A_HUMAN antibody
    • YL 8 antibody
    • YL8 antibody
    see all

Images

  • Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] - BSA and Azide free (ab240036)
    Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] - BSA and Azide free (ab240036)

    ab128913 (purified) at 1/20 immunoprecipitating Rab11A in A549 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) was used for detection at 1/10,000 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128913).

References

ab240036 has not yet been referenced specifically in any publications.

Publishing research using ab240036? Please let us know so that we can cite the reference in this datasheet.

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