Anti-RAB11B antibody (ab228954)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RAB11B antibody
See all RAB11B primary antibodies -
Description
Rabbit polyclonal to RAB11B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human RAB11B (internal sequence). The exact sequence is proprietary.
Database link: Q15907 -
Positive control
- IHC-P: Rat brain tissue; Human colon carcinoma tissue. WB: U-87 MG, SK-N-SH, IMR32, SK-N-AS, PC-12 and Rat2 whole cell extracts; Mouse brain lysate. ICC/IF: HeLa cells. IHC-Fr: Adult mouse cerebellum tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab228954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 24 kDa. | |
| IHC-Fr | 1/100 - 1/1000. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Post-translational
modificationsCitrullinated by PADI4. -
Cellular localization
Recycling endosome membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, phagosome membrane. Recruited to phagosomes containing S.aureus. - Information by UniProt
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Database links
- Entrez Gene: 420063 Chicken
- Entrez Gene: 532723 Cow
- Entrez Gene: 9230 Human
- Entrez Gene: 19326 Mouse
- Entrez Gene: 100513782 Pig
- Entrez Gene: 79434 Rat
- Entrez Gene: 707462 Rhesus monkey
- Omim: 604198 Human
see all -
Alternative names
- GTP-binding protein ypt3 antibody
- H YPT3 antibody
- RAB11B antibody
see all
Images
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Western blot - Anti-RAB11B antibody (ab228954)All lanes : Anti-RAB11B antibody (ab228954) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
Lane 3 : IMR32 whole cell lysate
Lane 4 : SK-N-AS whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 24 kDa12% SDS-PAGE gel.
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Western blot - Anti-RAB11B antibody (ab228954)Anti-RAB11B antibody (ab228954) at 1/1000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa12% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-RAB11B antibody (ab228954)2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RAB11B (green) using ab228954 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB11B antibody (ab228954)Paraffin-embedded human colon carcinoma tissue stained for RAB11B using ab228954 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Frozen sections) - Anti-RAB11B antibody (ab228954)Frozen-sectioned adult mouse cerebellum tissue stained for RAB11I using ab228954 at 1/1000 dilution in immunohistochemical analysis.
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Western blot - Anti-RAB11B antibody (ab228954)All lanes : Anti-RAB11B antibody (ab228954) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa12% SDS-PAGE gel.
References
ab228954 has not yet been referenced specifically in any publications.
