Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12719] to RAB11B
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-RAB11B antibody [EPR12719]
See all RAB11B primary antibodies
DescriptionRabbit monoclonal [EPR12719] to RAB11B
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RAB11B aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15907
- Fetal brain, Jurkat, SH-SY5Y, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 24 kDa.|
FunctionThe small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
modificationsCitrullinated by PADI4.
Cellular localizationRecycling endosome membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, phagosome membrane. Recruited to phagosomes containing S.aureus.
- Information by UniProt
- GTP-binding protein ypt3 antibody
- H YPT3 antibody
- RAB11B antibody
All lanes : Anti-RAB11B antibody [EPR12719] (ab176344) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Jurkat cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : C6 cell lysate
Lane 5 : Raw 264.7 cell lysate
Lane 6 : PC-12 cell lysate
Lane 7 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa
Western blot analysis on immunoprecipitation pellet from PC-12 cell lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176344 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
ab176344 has not yet been referenced specifically in any publications.