Recombinant
RabMAb

Anti-RAB11B antibody [EPR12719] (ab176344)

Overview

  • Product name
    Anti-RAB11B antibody [EPR12719]
    See all RAB11B primary antibodies
  • Description
    Rabbit monoclonal [EPR12719] to RAB11B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RAB11B aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15907

  • Positive control
    • Fetal brain, Jurkat, SH-SY5Y, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12719
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab176344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis.
    • Sequence similarities
      Belongs to the small GTPase superfamily. Rab family.
    • Post-translational
      modifications
      Citrullinated by PADI4.
    • Cellular localization
      Recycling endosome membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, phagosome membrane. Recruited to phagosomes containing S.aureus.
    • Information by UniProt
    • Database links
    • Alternative names
      • GTP-binding protein ypt3 antibody
      • H YPT3 antibody
      • RAB11B antibody
      • RAB11B member RAS oncogene family antibody
      • Ras-related protein Rab-11B antibody
      • RB11B_HUMAN antibody
      • YPT3 antibody
      see all

    Images

    • All lanes : Anti-RAB11B antibody [EPR12719] (ab176344) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : Raw 264.7 cell lysate
      Lane 6 : PC-12 cell lysate
      Lane 7 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 24 kDa

    • Western blot analysis on immunoprecipitation pellet from PC-12 cell lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176344 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    References

    ab176344 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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