Recombinant
RabMAb

Anti-RAB11B antibody [EPR12725] (ab175925)

Overview

  • Product name
    Anti-RAB11B antibody [EPR12725]
    See all RAB11B primary antibodies
  • Description
    Rabbit monoclonal [EPR12725] to RAB11B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RAB11B aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15907

  • Positive control
    • Fetal brain, 293T, U87-MG and Jurkat cell lysates; Jurkat cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12725
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab175925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 25 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis.
    • Sequence similarities
      Belongs to the small GTPase superfamily. Rab family.
    • Post-translational
      modifications
      Citrullinated by PADI4.
    • Cellular localization
      Recycling endosome membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, phagosome membrane. Recruited to phagosomes containing S.aureus.
    • Information by UniProt
    • Database links
    • Alternative names
      • GTP-binding protein ypt3 antibody
      • H YPT3 antibody
      • RAB11B antibody
      • RAB11B member RAS oncogene family antibody
      • Ras-related protein Rab-11B antibody
      • RB11B_HUMAN antibody
      • YPT3 antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from (1) fetal brain lysate or (2) 1X PBS (negative control) using ab175925 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Flow cytometric analysis of Jurkat cells labeling RAB11B with ab175925 at 1/10 dilution (red)  or a rabbit IgG (negative) (green).

    • All lanes : Anti-RAB11B antibody [EPR12725] (ab175925) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : 293T cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 25 kDa

    References

    ab175925 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (MDCK)
    Gel Running Conditions
    Reduced Denaturing (12% Gel (BioRad Fast Cast))
    Loading amount
    75000 cells
    Specification
    MDCK
    Blocking step
    TBST + 2.5% BSA + 1% milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.5% · Temperature: 25°C
    Username

    Ephrem Kassa

    Verified customer

    Submitted May 11 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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