Recombinant Anti-RAB11B antibody [EPR12725] (ab175925)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12725] to RAB11B
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RAB11B antibody [EPR12725]
See all RAB11B primary antibodies -
Description
Rabbit monoclonal [EPR12725] to RAB11B -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal brain, 293T, U87-MG and Jurkat cell lysates; Jurkat cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12725 -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 25 kDa.
|
IP |
1/10 - 1/100.
|
Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 25 kDa. |
IP
1/10 - 1/100. |
Target
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Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Post-translational
modificationsCitrullinated by PADI4. -
Cellular localization
Recycling endosome membrane. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, phagosome membrane. Recruited to phagosomes containing S.aureus. - Information by UniProt
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Database links
- Entrez Gene: 9230 Human
- Entrez Gene: 19326 Mouse
- Entrez Gene: 79434 Rat
- Omim: 604198 Human
- SwissProt: Q15907 Human
- SwissProt: P46638 Mouse
- SwissProt: O35509 Rat
- Unigene: 558403 Human
see all -
Alternative names
- GTP-binding protein ypt3 antibody
- H YPT3 antibody
- RAB11B antibody
see all
Images
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All lanes : Anti-RAB11B antibody [EPR12725] (ab175925) at 1/1000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa -
Western blot analysis on immunoprecipitation pellet from (1) fetal brain lysate or (2) 1X PBS (negative control) using ab175925 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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Intracellular flow cytometric analysis of Jurkat cells labeling RAB11B with ab175925 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab175925 has been referenced in 1 publication.
- Jehn U et al. α-Galactosidase a Deficiency in Fabry Disease Leads to Extensive Dysregulated Cellular Signaling Pathways in Human Podocytes. Int J Mol Sci 22:N/A (2021). PubMed: 34768768