Anti-RAB11FIP5 antibody (ab153843)
Key features and details
- Rabbit polyclonal to RAB11FIP5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RAB11FIP5 antibody
See all RAB11FIP5 primary antibodies -
Description
Rabbit polyclonal to RAB11FIP5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 112-338 of Human RAB11FIP5.
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Positive control
- 293T and A431 whole cell lysates; Human Hepatoma tissue; Mouse C2C12 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 70 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 70 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. -
Tissue specificity
Detected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 FIP-RBD domain. -
Domain
Binds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg(2+) rather than Ca(2+). -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Recycling endosome membrane. Mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 26056 Human
- Entrez Gene: 52055 Mouse
- Omim: 605536 Human
- SwissProt: Q9BXF6 Human
- SwissProt: Q8R361 Mouse
- Unigene: 24557 Human
- Unigene: 220334 Mouse
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Alternative names
- GAF 1 antibody
- Gaf-1 antibody
- GAF1 antibody
see all
Images
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All lanes : Anti-RAB11FIP5 antibody (ab153843) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 70 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue labeling RAB11FIP5 with ab153843 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse C2C12 xenograft labeling RAB11FIP5 with ab153843 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab153843 has not yet been referenced specifically in any publications.