Key features and details
- Rabbit polyclonal to RAB11FIP5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAB11FIP5 antibody
See all RAB11FIP5 primary antibodies
DescriptionRabbit polyclonal to RAB11FIP5
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cat, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192992 was affinity purified using an epitope specific to RAB11FIP5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab192992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 70 kDa.|
FunctionRab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane.
Tissue specificityDetected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas.
Sequence similaritiesContains 1 C2 domain.
Contains 1 FIP-RBD domain.
DomainBinds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg(2+) rather than Ca(2+).
modificationsPhosphorylated on serine and threonine residues.
Cellular localizationCytoplasm. Recycling endosome membrane. Mitochondrion membrane.
- Information by UniProt
- GAF 1 antibody
- Gaf-1 antibody
- GAF1 antibody
All lanes : Anti-RAB11FIP5 antibody (ab192992) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
ab192992 has not yet been referenced specifically in any publications.