Key features and details
- Mouse polyclonal to RAB11FIP5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAB11FIP5 antibody
See all RAB11FIP5 primary antibodies
DescriptionMouse polyclonal to RAB11FIP5
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein, corresponding to amino acids 1-652 of Human RAB11FIP5
- RAB11FIP5 transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab68947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 70 kDa).|
FunctionRab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane.
Tissue specificityDetected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas.
Sequence similaritiesContains 1 C2 domain.
Contains 1 FIP-RBD domain.
DomainBinds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg(2+) rather than Ca(2+).
modificationsPhosphorylated on serine and threonine residues.
Cellular localizationCytoplasm. Recycling endosome membrane. Mitochondrion membrane.
- Information by UniProt
- GAF 1 antibody
- Gaf-1 antibody
- GAF1 antibody
All lanes : Anti-RAB11FIP5 antibody (ab68947) at 1/500 dilution
Lane 1 : RAB11FIP5 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 70 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
ab68947 has not yet been referenced specifically in any publications.