Recombinant Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
![Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)](https://www.abcam.com/ps/products/256/ab256487/Images/ab256487-479214-256487.png "Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-RAB12 (phospho S106) antibody [MJF-R25-9]
See all RAB12 primary antibodies -
Description
Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Recombinant fragment
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Dot Blot: RAB12 (phospho S106) peptide. WB: Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment whole cell lysate, 293T transfected with RAB12 expression vector containing a myc-His-tag®, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This antibody was developed with support from The Michael J. Fox Foundation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R25-9 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
1/1000.
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| WB |
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
The antibody also reacts with unidentifiable proteins greater than 60 kDa. |
| Notes |
|---|
|
Dot blot
1/1000. |
|
WB
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). The antibody also reacts with unidentifiable proteins greater than 60 kDa. |
Target
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Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 201475 Human
- Entrez Gene: 19328 Mouse
- SwissProt: Q6IQ22 Human
- SwissProt: P35283 Mouse
- Unigene: 270074 Human
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Alternative names
- Putative Ras related protein Rab 12 antibody
- RAB12 antibody
- RAB12, member RAS oncogene family antibody
see all
Images
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Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)All lanes : Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate
Lane 2 : 293T transfected with RAB12 expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Exposure time: 120 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Observed Mw 30kDa
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![Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)](https://www.abcam.com/ps/products/256/ab256487/Images/ab256487-2-anti-rab12-phospho-S106--antibody-eprMJF25-9-western-blot-mef-mouse.jpg)
Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)All lanes : Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution
Lane 1 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment, whole cell lysate
Lane 2 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice treated with 100nM MLi-2 for 90 min, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Exposure time: 48 secondsThis antibody was used in the Mir, Tonelli et al. 2018 paper (PMID: 29743203).
MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).
The lysates were kindly provided by Professor Dario Alessi from University of Dundee.
The antibody also reacts with unidentifiable proteins greater than 60 kDa.
Blocking/Dilution buffer: AdvanBlock.
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![Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)](https://www.abcam.com/ps/products/256/ab256487/Images/ab256487-1-anti-rab12-phospho-S106--antibody-eprMJF25-9-dot-human.jpg)
Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487 at a 1/1000 dilution).
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary at 1/100,000 dilution.
Lane 1: RAB12 (phospho S106) peptide.
Lane 2: RAB12 non-phospho peptide.
Exposure time: 10 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)](https://www.abcam.com/ps/products/256/ab256487/Images/ab256487-3-benefits-of-recombinant-antibodies.png)
Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab256487 has been referenced in 1 publication.
- Kalogeropulou AF et al. Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity. Biochem J 477:4397-4423 (2020). PubMed: 33135724
