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    rab12-phospho-s106-antibody-mjf-r25-9-ab256487.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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RecombinantRabMAb

Recombinant Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
  • Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
  • Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
  • Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106)
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Recombinant fragment

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Overview

  • Product name

    Anti-RAB12 (phospho S106) antibody [MJF-R25-9]
    See all RAB12 primary antibodies
  • Description

    Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Dot Blot: RAB12 (phospho S106) peptide. WB: Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment whole cell lysate, 293T transfected with RAB12 expression vector containing a myc-His-tag®, whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This antibody was developed with support from The Michael J. Fox Foundation.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    MJF-R25-9
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-RAB12 (phospho S106) antibody [MJF-R25-9] - BSA and Azide free (ab256765)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab256487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
1/1000.
WB
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

The antibody also reacts with unidentifiable proteins greater than 60 kDa.

Notes
Dot blot
1/1000.
WB
1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

The antibody also reacts with unidentifiable proteins greater than 60 kDa.

Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Golgi apparatus membrane.
  • Target information above from: UniProt accession Q6IQ22 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 201475 Human
    • Entrez Gene: 19328 Mouse
    • SwissProt: Q6IQ22 Human
    • SwissProt: P35283 Mouse
    • Unigene: 270074 Human
    • Alternative names

      • Putative Ras related protein Rab 12 antibody
      • RAB12 antibody
      • RAB12, member RAS oncogene family antibody
      • RAB12_HUMAN antibody
      • Ras-related protein Rab-12 antibody
      see all

    Images

    • Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
      Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
      All lanes : Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution

      Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate
      Lane 2 : 293T transfected with RAB12 expression vector containing a myc-His-tag®, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 27 kDa


      Exposure time: 120 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Observed Mw 30kDa

    • Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
      Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
      All lanes : Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution

      Lane 1 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment, whole cell lysate
      Lane 2 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice treated with 100nM MLi-2 for 90 min, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 27 kDa


      Exposure time: 48 seconds


      This antibody was used in the Mir, Tonelli et al. 2018 paper (PMID: 29743203).

      MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).

      The lysates were kindly provided by Professor Dario Alessi from University of Dundee.

      The antibody also reacts with unidentifiable proteins greater than 60 kDa.

      Blocking/Dilution buffer: AdvanBlock.

    • Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
      Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)

      Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487 at a 1/1000 dilution).

      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary at 1/100,000 dilution.

      Lane 1: RAB12 (phospho S106) peptide.

      Lane 2: RAB12 non-phospho peptide.

      Exposure time: 10 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
      Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab256487? Please let us know so that we can cite the reference in this datasheet.

    ab256487 has been referenced in 1 publication.

    • Kalogeropulou AF  et al. Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity. Biochem J 477:4397-4423 (2020). PubMed: 33135724

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