Overview

  • Product name

    Anti-RAB12 (phospho S106) antibody [MJF-R25-9]
    See all RAB12 primary antibodies
  • Description

    Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human RAB12 aa 50-150 (phospho S106). The exact sequence is proprietary.
    Database link: Q6IQ22

  • Positive control

    • Dot Blot: RAB12 (phospho S106) peptide. WB: Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment whole cell lysate.
  • General notes

    This antibody was developed with support from The Michael J. Fox Foundation.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab256487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

The antibody also reacts with unidentifiable proteins greater than 60 kDa.

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution

      Lane 1 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment, whole cell lysate
      Lane 2 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice treated with 100nM MLi-2 for 90 min, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 27 kDa


      Exposure time: 48 seconds


      This antibody was used in the Mir, Tonelli et al. 2018 paper (PMID: 29743203).

      MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).

      The lysates were kindly provided by Professor Dario Alessi from University of Dundee.

      The antibody also reacts with unidentifiable proteins greater than 60 kDa.

      Blocking/Dilution buffer: AdvanBlock.

    • Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487 at a 1/1000 dilution).

      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary at 1/100,000 dilution.

      Lane 1: RAB12 (phospho S106) peptide.

      Lane 2: RAB12 non-phospho peptide.

      Exposure time: 10 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    References

    ab256487 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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