Recombinant Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-RAB12 (phospho S106) antibody [MJF-R25-9]
See all RAB12 primary antibodies -
Description
Rabbit monoclonal [MJF-R25-9] to RAB12 (phospho S106) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human RAB12 aa 50-150 (phospho S106). The exact sequence is proprietary.
Database link: Q6IQ22 -
Positive control
- Dot Blot: RAB12 (phospho S106) peptide. WB: Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment whole cell lysate.
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General notes
This antibody was developed with support from The Michael J. Fox Foundation.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R25-9 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab256487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | 1/1000. | |
| WB | 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). The antibody also reacts with unidentifiable proteins greater than 60 kDa. |
Target
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Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 201475 Human
- Entrez Gene: 19328 Mouse
- SwissProt: Q6IQ22 Human
- SwissProt: P35283 Mouse
- Unigene: 270074 Human
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Alternative names
- Putative Ras related protein Rab 12 antibody
- RAB12 antibody
- RAB12, member RAS oncogene family antibody
see all
Images
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![Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)](https://www.abcam.com/ps/products/256/ab256487/Images/ab256487-2-anti-rab12-phospho-S106--antibody-eprMJF25-9-western-blot-mef-mouse.jpg)
Western blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)All lanes : Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487) at 1/1000 dilution
Lane 1 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice without MLi-2 treatment, whole cell lysate
Lane 2 : Mouse embryonic fibroblasts from LRRK2[R1441C] knock in mice treated with 100nM MLi-2 for 90 min, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Exposure time: 48 secondsThis antibody was used in the Mir, Tonelli et al. 2018 paper (PMID: 29743203).
MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).
The lysates were kindly provided by Professor Dario Alessi from University of Dundee.
The antibody also reacts with unidentifiable proteins greater than 60 kDa.
Blocking/Dilution buffer: AdvanBlock.
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![Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)](https://www.abcam.com/ps/products/256/ab256487/Images/ab256487-1-anti-rab12-phospho-S106--antibody-eprMJF25-9-dot-human.jpg)
Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487)Dot Blot - Anti-RAB12 (phospho S106) antibody [MJF-R25-9] (ab256487 at a 1/1000 dilution).
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary at 1/100,000 dilution.
Lane 1: RAB12 (phospho S106) peptide.
Lane 2: RAB12 non-phospho peptide.
Exposure time: 10 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References
ab256487 has not yet been referenced specifically in any publications.
