Overview

  • Product name

    Anti-RAB13 antibody [EPR14110(2)]
    See all RAB13 primary antibodies
  • Description

    Rabbit monoclonal [EPR14110(2)] to RAB13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RAB13 aa 100-200. The exact sequence is proprietary.
    Database link: P51153

  • Positive control

    • WB: HEK-293, Caco-2, A431 and HeLa whole cell lysates; human fetal lung lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    Could participate in polarized transport, in the assembly and/or the activity of tight junctions.
  • Tissue specificity

    Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell junction > tight junction. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasmic tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell growth-inhibiting gene 4 protein antibody
    • GIG4 antibody
    • Growth inhibiting gene 4 protein antibody
    • RAB13 antibody
    • RAB13 member RAS oncogene family antibody
    • RAB13_HUMAN antibody
    • RAS associated protein RAB13 antibody
    • Ras related protein Rab13 antibody
    • Ras-related protein Rab-13 antibody
    see all

Images

  • All lanes : Anti-RAB13 antibody [EPR14110(2)] (ab205528) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : RAB13 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 23 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab205528 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab205528 was shown to specifically react with RAB13 in wild-type HAP1 cells as signal was lost in RAB13 knockout cells. Wild-type and RAB13 knockout samples were subjected to SDS-PAGE. Ab205528 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-RAB13 antibody [EPR14110(2)] (ab205528) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-RAB13 antibody [EPR14110(2)] (ab205528) at 1/2000 dilution + Human fetal lung lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab205528 has not yet been referenced specifically in any publications.

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