Product nameAnti-RAB14 antibody
See all RAB14 primary antibodies
DescriptionRabbit polyclonal to RAB14
Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Zebrafish
Synthetic peptide. This information is considered to be commercially sensitive.
- Raji cell lysate, Human kidney tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab28639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionMay be involved in vesicular trafficking and neurotransmitter release.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane.
- Information by UniProt
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ICC/IF image of ab28639 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28639, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-RAB14 antibody (ab28639) at 0.5 µg/ml + Raji cell lysate 10ug.
HRP conjugated anti-Rabbit IgG
Predicted band size: 24 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Human kidney tissue stained with ab28639 at X400 magnigication. The arrows point to epithelial cells of the renal tubule [positive label].
All lanes : Anti-RAB14 antibody (ab28639) at 2 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with blocking peptide
Lysates/proteins at 2 µg/ml per lane.
Predicted band size: 24 kDa
This product has been referenced in:
- Biswas K et al. Differentially Regulated Host Proteins Associated with Chronic Rhinosinusitis Are Correlated with the Sinonasal Microbiome. Front Cell Infect Microbiol 7:504 (2017). IHC-P ; Human . Read more (PubMed: 29270391) »
- Reed SE et al. A role for Rab14 in the endocytic trafficking of GLUT4 in 3T3-L1 adipocytes. J Cell Sci 126:1931-41 (2013). Mouse . Read more (PubMed: 23444368) »