Anti-Rab17 antibody (ab155135)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Rab17 antibody
See all Rab17 primary antibodies -
DescriptionRabbit polyclonal to Rab17
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-210 of Human Rab17 (Q9H0T7).
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Positive control
- NT2D1, IMR32, U87-MG and MCF7 whole cell lysates, MCF7 cells, H661 xenograft
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionMight be involved in transcellular transport.
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Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 64284 Human
- Omim: 602206 Human
- SwissProt: Q9H0T7 Human
- Unigene: 44278 Human
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Alternative names
- AW413472 antibody
- FLJ12538 antibody
- OTTHUMP00000164417 antibody
see all
Images
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Western blot - Anti-Rab17 antibody (ab155135)All lanes : Anti-Rab17 antibody (ab155135) at 1/2000 dilution
Lane 1 : U87-MG whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Rab17 antibody (ab155135)Immunofluorescent analysis of methanol-fixed MCF7 cells labeling Rab17 with ab155135 at 1/200. Image at the bottom costained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab17 antibody (ab155135)Immunohistochemical analysis of paraffin embedded H661 xenograft labeling Rab17 with ab155135 antibody at 1/500.
Protocols
References
ab155135 has not yet been referenced specifically in any publications.
