Overview

  • Product name
    Anti-Rab17 antibody
    See all Rab17 primary antibodies
  • Description
    Rabbit polyclonal to Rab17
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-210 of Human Rab17 (Q9H0T7).

  • Positive control
    • NT2D1, IMR32, U87-MG and MCF7 whole cell lysates, MCF7 cells, H661 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Might be involved in transcellular transport.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • AW413472 antibody
      • FLJ12538 antibody
      • OTTHUMP00000164417 antibody
      • OTTHUMP00000202805 antibody
      • Rab 17 antibody
      • Rab17 antibody
      • RAB17 member RAS oncogene family antibody
      • RAB17_HUMAN antibody
      • Ras related protein Rab 17 antibody
      • Ras related protein Rab17 antibody
      • Ras-related protein Rab-17 antibody
      see all

    Images

    • Western blot - Anti-Rab17 antibody (ab155135)
      Western blot - Anti-Rab17 antibody (ab155135)
      All lanes : Anti-Rab17 antibody (ab155135) at 1/2000 dilution

      Lane 1 : U87-MG whole cell lysate
      Lane 2 : MCF7 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 23 kDa



      12% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-Rab17 antibody (ab155135)
      Immunocytochemistry/ Immunofluorescence - Anti-Rab17 antibody (ab155135)
      Immunofluorescent analysis of methanol-fixed MCF7 cells labeling Rab17 with ab155135 at 1/200. Image at the bottom costained with Hoechst 33342.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab17 antibody (ab155135)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab17 antibody (ab155135)
      Immunohistochemical analysis of paraffin embedded H661 xenograft labeling Rab17 with ab155135 antibody at 1/500.

    References

    ab155135 has not yet been referenced specifically in any publications.

    Publishing research using ab155135? Please let us know so that we can cite the reference in this datasheet.

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