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    rab17-antibody-ab155135.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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Anti-Rab17 antibody (ab155135)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-Rab17 antibody (ab155135)
  • Western blot - Anti-Rab17 antibody (ab155135)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab17 antibody (ab155135)

Key features and details

  • Rabbit polyclonal to Rab17
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant mouse Rab17 protein (ab90777)

View more associated products

Overview

  • Product name

    Anti-Rab17 antibody
    See all Rab17 primary antibodies
  • Description

    Rabbit polyclonal to Rab17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-210 of Human Rab17 (Q9H0T7).

  • Positive control

    • NT2D1, IMR32, U87-MG and MCF7 whole cell lysates, MCF7 cells, H661 xenograft
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant mouse Rab17 protein (ab90777)
  • Related Products

    • Recombinant Human Rab17 protein (ab109833)
    • Recombinant mouse Rab17 protein (ab90777)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Might be involved in transcellular transport.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q9H0T7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64284 Human
    • Omim: 602206 Human
    • SwissProt: Q9H0T7 Human
    • Unigene: 44278 Human
    • Alternative names

      • AW413472 antibody
      • FLJ12538 antibody
      • OTTHUMP00000164417 antibody
      • OTTHUMP00000202805 antibody
      • Rab 17 antibody
      • Rab17 antibody
      • RAB17 member RAS oncogene family antibody
      • RAB17_HUMAN antibody
      • Ras related protein Rab 17 antibody
      • Ras related protein Rab17 antibody
      • Ras-related protein Rab-17 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Rab17 antibody (ab155135)
      Immunocytochemistry/ Immunofluorescence - Anti-Rab17 antibody (ab155135)
      Immunofluorescent analysis of methanol-fixed MCF7 cells labeling Rab17 with ab155135 at 1/200. Image at the bottom costained with Hoechst 33342.
    • Western blot - Anti-Rab17 antibody (ab155135)
      Western blot - Anti-Rab17 antibody (ab155135)
      All lanes : Anti-Rab17 antibody (ab155135) at 1/2000 dilution

      Lane 1 : U87-MG whole cell lysate
      Lane 2 : MCF7 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 23 kDa



      12% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab17 antibody (ab155135)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab17 antibody (ab155135)
      Immunohistochemical analysis of paraffin embedded H661 xenograft labeling Rab17 with ab155135 antibody at 1/500.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab155135? Please let us know so that we can cite the reference in this datasheet.

    ab155135 has not yet been referenced specifically in any publications.

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