Anti-Rab17 antibody (ab155135)
Key features and details
- Rabbit polyclonal to Rab17
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rab17 antibody
See all Rab17 primary antibodies -
Description
Rabbit polyclonal to Rab17 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-210 of Human Rab17 (Q9H0T7).
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Positive control
- NT2D1, IMR32, U87-MG and MCF7 whole cell lysates, MCF7 cells, H661 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Might be involved in transcellular transport. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 64284 Human
- Omim: 602206 Human
- SwissProt: Q9H0T7 Human
- Unigene: 44278 Human
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Alternative names
- AW413472 antibody
- FLJ12538 antibody
- OTTHUMP00000164417 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Rab17 antibody (ab155135)Immunofluorescent analysis of methanol-fixed MCF7 cells labeling Rab17 with ab155135 at 1/200. Image at the bottom costained with Hoechst 33342.
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Western blot - Anti-Rab17 antibody (ab155135)All lanes : Anti-Rab17 antibody (ab155135) at 1/2000 dilution
Lane 1 : U87-MG whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab17 antibody (ab155135)Immunohistochemical analysis of paraffin embedded H661 xenograft labeling Rab17 with ab155135 antibody at 1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155135 has not yet been referenced specifically in any publications.