Anti-Rab17 antibody (ab155135)

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Overview

  • Product name

    Anti-Rab17 antibody
    See all Rab17 primary antibodies

  • Description

    Rabbit polyclonal to Rab17

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-210 of Human Rab17 (Q9H0T7).

  • Positive control

    • NT2D1, IMR32, U87-MG and MCF7 whole cell lysates, MCF7 cells, H661 xenograft

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Might be involved in transcellular transport.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • AW413472 antibody
      • FLJ12538 antibody
      • OTTHUMP00000164417 antibody
      • OTTHUMP00000202805 antibody
      • Rab 17 antibody
      • Rab17 antibody
      • RAB17 member RAS oncogene family antibody
      • RAB17_HUMAN antibody
      • Ras related protein Rab 17 antibody
      • Ras related protein Rab17 antibody
      • Ras-related protein Rab-17 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Rab17 antibody (ab155135)
      Immunocytochemistry/ Immunofluorescence - Anti-Rab17 antibody (ab155135)
      Immunofluorescent analysis of methanol-fixed MCF7 cells labeling Rab17 with ab155135 at 1/200. Image at the bottom costained with Hoechst 33342.
    • Western blot - Anti-Rab17 antibody (ab155135)
      Western blot - Anti-Rab17 antibody (ab155135)
      All lanes : Anti-Rab17 antibody (ab155135) at 1/2000 dilution

      Lane 1 : U87-MG whole cell lysate
      Lane 2 : MCF7 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 23 kDa



      12% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab17 antibody (ab155135)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab17 antibody (ab155135)
      Immunohistochemical analysis of paraffin embedded H661 xenograft labeling Rab17 with ab155135 antibody at 1/500.

    References

    ab155135 has not yet been referenced specifically in any publications.

    Publishing research using ab155135? Please let us know so that we can cite the reference in this datasheet.

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