Anti-Rab18 antibody (ab224466)

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Overview

  • Product name

    Anti-Rab18 antibody
    See all Rab18 primary antibodies

  • Description

    Rabbit polyclonal to Rab18

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Orangutan

  • Immunogen

    Recombinant fragment corresponding to Human Rab18 aa 87-205.
    Sequence:

    VYDVTRRDTFVKLDNWLNELETYCTRNDIVNMLVGNKIDKENREVDRNEG LKFARKHSMLFIEASAKTCDGVQCAFEELVEKIIQTPGLWESENQNKGVK LSHREEGQGGGACGGYCSV


    Database link: Q9NP72

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human prostate tissue. WB: Rab18 overexpression HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab224466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 23 kDa.

Target

Images

  • Western blot - Anti-Rab18 antibody (ab224466)
    Western blot - Anti-Rab18 antibody (ab224466)
    All lanes : Anti-Rab18 antibody (ab224466) at 1/100 dilution

    Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : Rab18 overexpression HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) co-expressed with a C-terminal myc-DDK tag, cell lysate

    Developed using the ECL technique.

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (ab224466)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab18 antibody (ab224466)

    Paraffin-embedded human prostate tissue stained for Rab18 using ab224466 at 1/200 dilution in immunohistochemical analysis.

References

ab224466 has not yet been referenced specifically in any publications.

Publishing research using ab224466? Please let us know so that we can cite the reference in this datasheet.

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