Overview

  • Product name

  • Description

    Rabbit polyclonal to Rab1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Chicken, Dog, Pig, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Rab1A (internal sequence). The exact sequence is proprietary.
    Database link: P62820

  • Positive control

    • WB: Rab1A transfected HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.

Target

  • Function

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Post-translational
    modifications

    Phosphorylated by CDK1 kinase during mitosis.
    Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB.
  • Cellular localization

    Golgi apparatus. Endoplasmic reticulum. Early endosome. Cytoplasm, cytosol. Membrane. Melanosome. Alternates between membrane-associated and cytosolic forms.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTP binding protein RAB 1A antibody
    • mKIAA3012 antibody
    • RAB 1 antibody
    • Rab 1A antibody
    • RAB1 antibody
    • RAB1, member RAS oncogene family antibody
    • Rab1A antibody
    • RAB1A member RAS oncogene family antibody
    • RAB1A_HUMAN antibody
    • Ras related protein Rab 1A antibody
    • Ras-associated protein RAB1 antibody
    • Ras-related protein Rab-1A antibody
    • YPT1 antibody
    • YPT1 related protein antibody
    • YPT1-related protein antibody
    see all

Images

  • All lanes : Anti-Rab1A antibody (ab228354) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Rab1A transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Predicted band size: 22 kDa



    12% SDS-PAGE gel.

References

ab228354 has not yet been referenced specifically in any publications.

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