Anti-RAB1B antibody (ab234717)
Key features and details
- Rabbit polyclonal to RAB1B
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAB1B antibody -
Description
Rabbit polyclonal to RAB1B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant fragment corresponding to Human RAB1B aa 100 to the C-terminus.
Database link: Q9H0U4 -
Positive control
- ICC/IF: U-251 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >955 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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Notes |
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ICC/IF
1/50 - 1/200. |
Target
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Function
Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Post-translational
modificationsPrenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active. -
Cellular localization
Membrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 81876 Human
- Entrez Gene: 76308 Mouse
- Entrez Gene: 100126191 Rat
- Omim: 612565 Human
- SwissProt: Q2HJH2 Cow
- SwissProt: Q4R8X3 Cynomolgus monkey
- SwissProt: Q9H0U4 Human
- SwissProt: Q9D1G1 Mouse
see all -
Alternative names
- RAB1B antibody
- RAB1B member RAS oncogene family antibody
- RAB1B_HUMAN antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234717 has not yet been referenced specifically in any publications.