Anti-Rab20 antibody (ab197209)
Key features and details
- Rabbit polyclonal to Rab20
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rab20 antibody
See all Rab20 primary antibodies -
Description
Rabbit polyclonal to Rab20 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Rab20 aa 1 to the C-terminus. Genbank: BC026025
Database link: Q9NX57 -
Positive control
- HeLa and MCF7 cell lysates. Human prostate cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Add 200 µL of sterile distilled water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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WB |
1/200 - 1/1000. Predicted molecular weight: 26 kDa.
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Notes |
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IHC-P
1/100 - 1/500. |
WB
1/200 - 1/1000. Predicted molecular weight: 26 kDa. |
Target
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Function
Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. -
Tissue specificity
Low or absent expression in normal pancreas and stronger expression in 15 of 18 exocrine pancreatic adenocarcinomas (at protein level). -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Golgi apparatus. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane. Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules (By similarity). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). - Information by UniProt
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Database links
- Entrez Gene: 55647 Human
- SwissProt: Q9NX57 Human
- Unigene: 743563 Human
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Alternative names
- FLJ20429 antibody
- RAB20 antibody
- RAB20 member RAS oncogene family antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab197209 has been referenced in 1 publication.
- Tan X et al. RAB20 Promotes Proliferation via G2/M Phase through the Chk1/cdc25c/cdc2-cyclinB1 Pathway in Penile Squamous Cell Carcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 35267417