
Recombinant Anti-RAB22A antibody [EPR9486] (ab137093)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-RAB22A antibody [EPR9486]
See all RAB22A primary antibodies -
Description
Rabbit monoclonal [EPR9486] to RAB22A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human RAB22A aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: MCF-7 and BxPC-3 cell lysate; fetal kidney and fetal heart tissue lysates ICC/IF: MCF-7 cells. IP: MCF-7 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9486 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab137093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Predicted molecular weight: 22 kDa. | |
ICC/IF | 1/100. For unpurified use at 1/250 - 1/500. |
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IP | 1/10 - 1/100. |
Target
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Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Endosome membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57403 Human
- Entrez Gene: 19334 Mouse
- Entrez Gene: 366265 Rat
- Omim: 612966 Human
- SwissProt: Q9UL26 Human
- SwissProt: P35285 Mouse
- Unigene: 529044 Human
- Unigene: 275304 Mouse
see all -
Alternative names
- 3732413A17Rik antibody
- AI662177 antibody
- AW319644 antibody
see all
Images
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All lanes : Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa -
Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa -
All lanes : Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution (unpurified)
Lane 1 : MCF-7 lysate
Lane 2 : Fetal kidney lysate
Lane 3 : BxPC-3 lysate
Lane 4 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 22 kDa -
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling RAB22A with purified ab137093 at 1/100 dilution (7.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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ab137093 (purified) at 1/40 dilution (2ug) immunoprecipitating RAB22A in MCF7 whole cell lysates.
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates 10ug
Lane 2 (+): ab137093 & MCF7 whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137093 in MCF7 whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Protocols
Datasheets and documents
References
This product has been referenced in:
- Fang Z et al. MicroRNA-193b acts as a tumor suppressor in colon cancer progression via targeting RAB22A. Exp Ther Med 17:3921-3928 (2019). Read more (PubMed: 31007734) »
- Zhang L et al. Hypoxia preconditioned renal tubular epithelial cell-derived extracellular vesicles alleviate renal ischaemia-reperfusion injury mediated by the HIF-1a/Rab22 pathway and potentially affected by microRNAs. Int J Biol Sci 15:1161-1176 (2019). Read more (PubMed: 31223277) »