Recombinant
RabMAb

Recombinant Anti-RAB22A antibody [EPR9486] (ab137093)

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Overview

  • Product name

    Anti-RAB22A antibody [EPR9486]
    See all RAB22A primary antibodies

  • Description

    Rabbit monoclonal [EPR9486] to RAB22A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human RAB22A aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: MCF-7 and BxPC-3 cell lysate; fetal kidney and fetal heart tissue lysates ICC/IF: MCF-7 cells. IP: MCF-7 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100.

For unpurified use at 1/250 - 1/500.
IP 1/10 - 1/100.

Target

Images

  • Western blot - Anti-RAB22A antibody [EPR9486] (ab137093)
    Western blot - Anti-RAB22A antibody [EPR9486] (ab137093)
    All lanes : Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution (Purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Western blot - Anti-RAB22A antibody [EPR9486] (ab137093)
    Western blot - Anti-RAB22A antibody [EPR9486] (ab137093)
    Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Western blot - Anti-RAB22A antibody [EPR9486] (ab137093)
    Western blot - Anti-RAB22A antibody [EPR9486] (ab137093)
    All lanes : Anti-RAB22A antibody [EPR9486] (ab137093) at 1/1000 dilution (unpurified)

    Lane 1 : MCF-7 lysate
    Lane 2 : Fetal kidney lysate
    Lane 3 : BxPC-3 lysate
    Lane 4 : Fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (ab137093)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (ab137093)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling RAB22A with purified ab137093 at 1/100 dilution (7.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (ab137093)
    Immunoprecipitation - Anti-RAB22A antibody [EPR9486] (ab137093)

    ab137093 (purified) at 1/40 dilution (2ug) immunoprecipitating RAB22A in MCF7 whole cell lysates.
    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab137093 & MCF7 whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137093 in MCF7 whole cell lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (ab137093)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB22A antibody [EPR9486] (ab137093)
    Immunocytochemistry/ Immunofluorescence analysis of MCF-7 cells labelling RAB22A with ab137093 (unpurified) at 1/250 dilution.

References

This product has been referenced in:

  • Fang Z  et al. MicroRNA-193b acts as a tumor suppressor in colon cancer progression via targeting RAB22A. Exp Ther Med 17:3921-3928 (2019). Read more (PubMed: 31007734) »
  • Zhang L  et al. Hypoxia preconditioned renal tubular epithelial cell-derived extracellular vesicles alleviate renal ischaemia-reperfusion injury mediated by the HIF-1a/Rab22 pathway and potentially affected by microRNAs. Int J Biol Sci 15:1161-1176 (2019). Read more (PubMed: 31223277) »
See all 5 Publications for this product

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