Key features and details
- Rabbit polyclonal to RAB23
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RAB23 antibody
See all RAB23 primary antibodies
DescriptionRabbit polyclonal to RAB23
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human RAB23 aa 1-237.
MLEEDMEVAIKMVVVGNGAVGKSSMIQRYCKGIFTKDYKKTIGVDFLERQ IQVNDEDVRLMLWDTAGQEEFDAITKAYYRGAQACVLVFSTTDRESFEAV SSWREKVVAEVGDIPTVLVQNKIDLLDDSCIKNEEAEALAKRLKLRFYRT SVKEDLNVNEVFKYLAEKYLQKLKQQIAEDPELTHSSSNKIGVFNTSGGS HSGQNSGTLNGGDVINLRPNKQRTKKNRNPFSSCSIP
Database link: Q9ULC3
- WB: Mouse ocular, kidney, lung and brain tissue lysate; Rat brain lysate; NIH/3T3, MCF7 and HeLa whole cell lysate (ab150035). IHC-P: Human liver cancer and adrenal gland tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- Mouse lung normal tissue lysate - total protein (ab29297)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- MCF7 whole cell lysate (ab3871)
- Mouse brain tissue lysate - total protein (ab4022)
- NIH 3T3 whole cell lysate (ab7179)
Our Abpromise guarantee covers the use of ab230200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 27 kDa.|
Involvement in diseaseDefects in RAB23 are the cause of acrocephalopolysyndactyly type 2 (ACPS2) [MIM:201000]. A syndrome characterized by craniosynostosis, polysyndactyly, obesity, and cardiac defects.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane.
- Information by UniProt
- DKFZp781H0695 antibody
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All lanes : Anti-RAB23 antibody (ab230200) at 1/1000 dilution
Lane 1 : Mouse ocular tissue
Lane 2 : Rat brain tissue
Lane 3 : Mouse kidney tissue
Lane 4 : Mouse lung tissue
Lane 5 : Mouse brain tissue
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 7 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 8 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 27 kDa
Paraffin-embedded human adrenal gland tissue stained for RAB23 using ab230200 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human liver cancer tissue stained for RAB23 using ab230200 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230200 has been referenced in 1 publication.
- Zhao T et al. Rab23 contributes to the progression of colorectal cancer via protein kinase B and extracellular signal-regulated kinase signaling pathways. Oncol Lett 18:1793-1799 (2019). PubMed: 31423247