Key features and details
- Goat polyclonal to RAB27A + RAB27B - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Dog, Human, Monkey
- Isotype: IgG
Product nameAnti-RAB27A + RAB27B antibody - C-terminal
DescriptionGoat polyclonal to RAB27A + RAB27B - C-terminal
SpecificityThis antibody detects total RAB27A + RAB27B levels
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, Monkey
This product was produced with the following immunogens:
Recombinant fragment within Mouse RAB27A + RAB27B aa 120-221 (C terminal). The exact sequence is proprietary. RAB27A, produced in E coli
RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE LAEKYGIPYF ETSAANGTNI SHAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHTSADQL SEEKEKGLCG C
Database link: Q9ERI2
Recombinant fragment within Mouse RAB27A + RAB27B aa 120-218 (C terminal). The exact sequence is proprietary. RAB27B, produced in E coli
Sequence: RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE LAEKYGIPYF ETSAATGQNV EKSVETLLDL IMKRMEKCVE KTQVPDTVNG GNSGKLDGEK PAEKKCAC
Database link: Q99P58
- Melan-Ink; AtT 20 cell lysates; B65-RPE07 cells; primary RPE cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab192673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 25, 24 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
All lanes : Anti-RAB27A + RAB27B antibody - C-terminal (ab192673) at 1/500 dilution
Lane 1 : Mela-Ink cell lysate
Lane 2 : AtT 20 cell lysate
All lanes : HRP conjugated rabbit anti goat at 1/10000 dilution
Predicted band size: 25, 24 kDa
Immunofluorescence analysis of primary RPE cells labeling RAB27A + RAB27B using ab192673 at 1/50 dilution.
Immunofluorescence analysis of B6-RPE07 cells labeling RAB27A + RAB27B using ab192673 at 1/50 dilution.
ab192673 has not yet been referenced specifically in any publications.