Overview

  • Product name

    Anti-RAB27A + RAB27B antibody - C-terminal
  • Description

    Goat polyclonal to RAB27A + RAB27B - C-terminal
  • Host species

    Goat
  • Specificity

    This antibody detects total RAB27A + RAB27B levels
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human, Monkey
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment within Mouse RAB27A + RAB27B aa 120-221 (C terminal). The exact sequence is proprietary. RAB27A, produced in E coli
    Sequence:

    RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE LAEKYGIPYF ETSAANGTNI SHAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHTSADQL SEEKEKGLCG C


    Database link: Q9ERI2

    Recombinant fragment within Mouse RAB27A + RAB27B aa 120-218 (C terminal). The exact sequence is proprietary. RAB27B, produced in E coli
    Sequence: RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE LAEKYGIPYF ETSAATGQNV EKSVETLLDL IMKRMEKCVE KTQVPDTVNG GNSGKLDGEK PAEKKCAC
    Database link: Q99P58

  • Positive control

    • Melan-Ink; AtT 20 cell lysates; B65-RPE07 cells; primary RPE cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 25, 24 kDa.
ICC/IF Use at an assay dependent concentration.

Images

  • All lanes : Anti-RAB27A + RAB27B antibody - C-terminal (ab192673) at 1/500 dilution

    Lane 1 : Mela-Ink cell lysate
    Lane 2 : AtT 20 cell lysate

    Secondary
    All lanes : HRP conjugated rabbit anti goat at 1/10000 dilution

    Predicted band size: 25, 24 kDa

  • Immunofluorescence analysis of primary RPE cells labeling RAB27A + RAB27B using ab192673 at 1/50 dilution.

  • Immunofluorescence analysis of B6-RPE07 cells labeling RAB27A + RAB27B using ab192673 at 1/50 dilution.

References

ab192673 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192673.
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