Product nameAnti-RAB27A + RAB27B antibody - C-terminal
DescriptionGoat polyclonal to RAB27A + RAB27B - C-terminal
SpecificityThis antibody detects total RAB27A + RAB27B levels
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human, Monkey
This product was produced with the following immunogens:
Recombinant fragment within Mouse RAB27A + RAB27B aa 120-221 (C terminal). The exact sequence is proprietary. RAB27A, produced in E coli
RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE LAEKYGIPYF ETSAANGTNI SHAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHTSADQL SEEKEKGLCG C
Database link: Q9ERI2
Recombinant fragment within Mouse RAB27A + RAB27B aa 120-218 (C terminal). The exact sequence is proprietary. RAB27B, produced in E coli
Sequence: RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE LAEKYGIPYF ETSAATGQNV EKSVETLLDL IMKRMEKCVE KTQVPDTVNG GNSGKLDGEK PAEKKCAC
Database link: Q99P58
- Melan-Ink; AtT 20 cell lysates; B65-RPE07 cells; primary RPE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab192673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 25, 24 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
All lanes : Anti-RAB27A + RAB27B antibody - C-terminal (ab192673) at 1/500 dilution
Lane 1 : Mela-Ink cell lysate
Lane 2 : AtT 20 cell lysate
All lanes : HRP conjugated rabbit anti goat at 1/10000 dilution
Predicted band size: 25, 24 kDa
Immunofluorescence analysis of primary RPE cells labeling RAB27A + RAB27B using ab192673 at 1/50 dilution.
Immunofluorescence analysis of B6-RPE07 cells labeling RAB27A + RAB27B using ab192673 at 1/50 dilution.
ab192673 has not yet been referenced specifically in any publications.