Overview

  • Product name

    Anti-RAB28 antibody
  • Description

    Mouse polyclonal to RAB28
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Human
  • Immunogen

    Full length RAB28 protein (Human)

  • Positive control

    • transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa).

Target

  • Relevance

    RAB28 belongs to the small GTPase superfamily. There are two isoforms. Isoform S is detected in most tissues investigated (cortex, liver, kidney, skeletal muscle, adipose tissue, testis and urothelium), whereas isoform L is predominant in testis.
  • Cellular localization

    Cell membrane; Lipid-anchor; Cytoplasmic side.
  • Database links

  • Alternative names

    • MGC41862 antibody
    • Rab26 antibody
    • RAB28, member RAS oncogene family antibody
    • Ras related protein Rab 28 antibody
    • Ras related protein Rab28 antibody
    see all

Images

  • All lanes : Anti-RAB28 antibody (ab67085) at 1/500 dilution

    Lane 1 : RAB28 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 26 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?

References

ab67085 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

ab67077 is actually a fragment of Rab28. I have updated the datasheet. Apologies for the confusion. This is why different bands sizes for immunogen transfected samples are seen. Both antibodies should be able to recognize both isoforms if present.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up