Anti-RAB28 antibody (ab67085)

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Overview

  • Product name

    Anti-RAB28 antibody

  • Description

    Mouse polyclonal to RAB28

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Human

  • Immunogen

    Full length RAB28 protein (Human)

  • Positive control

    • transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 26 kDa).

Target

  • Relevance

    RAB28 belongs to the small GTPase superfamily. There are two isoforms. Isoform S is detected in most tissues investigated (cortex, liver, kidney, skeletal muscle, adipose tissue, testis and urothelium), whereas isoform L is predominant in testis.

  • Cellular localization

    Cell membrane; Lipid-anchor; Cytoplasmic side.

  • Database links

    • Alternative names

      • MGC41862 antibody
      • Rab26 antibody
      • RAB28, member RAS oncogene family antibody
      • Ras related protein Rab 28 antibody
      • Ras related protein Rab28 antibody
      see all

    Images

    • Western blot - Anti-RAB28 antibody (ab67085)
      Western blot - Anti-RAB28 antibody (ab67085)
      All lanes : Anti-RAB28 antibody (ab67085) at 1/500 dilution

      Lane 1 : RAB28 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 26 kDa
      Observed band size: 25 kDa
      why is the actual band size different from the predicted?

    References

    ab67085 has not yet been referenced specifically in any publications.

    Publishing research using ab67085? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    We plan to have a study on Rab28 GTPase and would like to purchase its antibody from Abcam. In your website, there are two kinds of Rab28 antibodies: ab67077 and ab67085. They have very close descriptions. If ab67077, which predicted molecular weight 12 kDa, is the isoform S? On the other hand, if ab67085, which predicted molecular weight 26KD, is the isoform L? These isoforms of Rab28 have very different tissue distributions,

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    Answer

    ab67077 is actually a fragment of Rab28. I have updated the datasheet. Apologies for the confusion. This is why different bands sizes for immunogen transfected samples are seen. Both antibodies should be able to recognize both isoforms if present.

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