Overview

  • Product name

    Anti-RAB29 antibody [MJF-R30-104]
    See all RAB29 primary antibodies
  • Description

    Rabbit monoclonal [MJF-R30-104] to RAB29
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RAB29 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: O14966

  • Positive control

    • WB: Wild type A549, A549, HEK293T cells and HEK293T cells lysates. IP: A549 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab256527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 23 kDa.
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Rab GTPase key regulator in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans-Golgi network structure. Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Rab family.
    • Post-translational
      modifications

      In case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells (PubMed:22042847).
    • Cellular localization

      Cell membrane. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network. Vacuole. Cytoplasm, cytoskeleton. Colocalizes with LRRK2 along tubular structures emerging from Golgi apparatus (By similarity). Colocalizes with GM130 at the Golgi apparatus. Colocalizes with dynamic tubules emerging from and retracting to the Golgi apparatus. Colocalizes with TGN46 at the trans-Golgi network (TGN). In Salmonella enterica serovar Typhi (S.Typhi) infected epithelial cells, is recruited and colocalized with both S.Typhi-containing vacuoles and dynamic tubules as well as those emerging from the vacuole toward the cell periphery.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp686P1051 antibody
      • Rab 7 like protein 1 antibody
      • RAB 7L antibody
      • Rab-7-like protein 1 antibody
      • RAB29 antibody
      • Rab7 like protein 1 antibody
      • RAB7 member RAS oncogene family like 1 antibody
      • RAB7L antibody
      • RAB7L_HUMAN antibody
      • Ras related protein Rab 7L1 antibody
      • Ras related protein Rab7L1 antibody
      • Ras-related protein Rab-29 antibody
      • Ras-related protein Rab-7L1 antibody
      see all

    Images

    • All lanes : Anti-RAB29 antibody [MJF-R30-104] (ab256527) at 1/4000 dilution

      Lane 1 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with non-target siRNA control whole cell lysate 15ug
      Lane 2 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with siRNA against Rab29 whole cell lysate 15ug

      Secondary
      All lanes : IR Dye 800CW Goat anti-rabbit IgG (#926-32211) at 1/10000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa



      This image was kindly provided by Dr. Mark Cookson, NIH

      Blocking/Diluting buffer and concentration: 50% TBST, 50% Odyssey Blocking buffer 

       

    • All lanes : Anti-RAB29 antibody [MJF-R30-104] (ab256527) at 1/1000 dilution

      Lane 1 : Wild type A549 (human lung carcinoma epithelial cell) whole cell lysate 20ug
      Lane 2 : A549 (human lung carcinoma epithelial cell) Rab29 KO whole cell lysate 20ug

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa



      The lysates were kindly provided by Dr. Dario Alessi, University of Dundee.

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

      Exposure Time: 59 seconds

    • RAB29 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab256527 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256527 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug

      Lane 2: ab256527 IP in A549 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256527 in A549 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST

      Exposure time: 30 seconds

       

    References

    ab256527 has not yet been referenced specifically in any publications.

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