Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R30-104] to RAB29
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Product nameAnti-RAB29 antibody [MJF-R30-104]
See all RAB29 primary antibodies
DescriptionRabbit monoclonal [MJF-R30-104] to RAB29
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human RAB29 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: O14966
- WB: Wild type A549, A549, HEK293T cells and HEK293T cells lysates. IP: A549 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab256527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 23 kDa.|
FunctionRab GTPase key regulator in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans-Golgi network structure. Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
modificationsIn case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells (PubMed:22042847).
Cellular localizationCell membrane. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network. Vacuole. Cytoplasm, cytoskeleton. Colocalizes with LRRK2 along tubular structures emerging from Golgi apparatus (By similarity). Colocalizes with GM130 at the Golgi apparatus. Colocalizes with dynamic tubules emerging from and retracting to the Golgi apparatus. Colocalizes with TGN46 at the trans-Golgi network (TGN). In Salmonella enterica serovar Typhi (S.Typhi) infected epithelial cells, is recruited and colocalized with both S.Typhi-containing vacuoles and dynamic tubules as well as those emerging from the vacuole toward the cell periphery.
- Information by UniProt
- DKFZp686P1051 antibody
- Rab 7 like protein 1 antibody
- RAB 7L antibody
All lanes : Anti-RAB29 antibody [MJF-R30-104] (ab256527) at 1/4000 dilution
Lane 1 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with non-target siRNA control whole cell lysate 15ug
Lane 2 : HEK293T cells (human embryonic kidney epithelial cell) transiently transfected with siRNA against Rab29 whole cell lysate 15ug
All lanes : IR Dye 800CW Goat anti-rabbit IgG (#926-32211) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
This image was kindly provided by Dr. Mark Cookson, NIH
Blocking/Diluting buffer and concentration: 50% TBST, 50% Odyssey Blocking buffer
All lanes : Anti-RAB29 antibody [MJF-R30-104] (ab256527) at 1/1000 dilution
Lane 1 : Wild type A549 (human lung carcinoma epithelial cell) whole cell lysate 20ug
Lane 2 : A549 (human lung carcinoma epithelial cell) Rab29 KO whole cell lysate 20ug
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
The lysates were kindly provided by Dr. Dario Alessi, University of Dundee.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure Time: 59 seconds
RAB29 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab256527 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256527 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab256527 IP in A549 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256527 in A549 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 30 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab256527 has not yet been referenced specifically in any publications.