Recombinant Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3] (ab291075)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R40-R3-H3] to RAB29 (phospho S72)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3]
See all RAB29 primary antibodies -
Description
Rabbit monoclonal [MJF-R40-R3-H3] to RAB29 (phospho S72) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Whole cell lysates of HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 expression vector containing a HA-tag. RAB29 expression vector contained either the single T71A mutation or both T71A and S72A mutations. Cells were either treated or not treated with 200 µM MLi-2 for 1.5h. All variations were HA-tagged.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This antibody was developed with support from The Michael J. Fox Foundation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R40-R3-H3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab291075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
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Notes |
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WB
1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Rab GTPase key regulator in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans-Golgi network structure. Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Post-translational
modificationsIn case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells (PubMed:22042847). -
Cellular localization
Cell membrane. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network. Vacuole. Cytoplasm, cytoskeleton. Colocalizes with LRRK2 along tubular structures emerging from Golgi apparatus (By similarity). Colocalizes with GM130 at the Golgi apparatus. Colocalizes with dynamic tubules emerging from and retracting to the Golgi apparatus. Colocalizes with TGN46 at the trans-Golgi network (TGN). In Salmonella enterica serovar Typhi (S.Typhi) infected epithelial cells, is recruited and colocalized with both S.Typhi-containing vacuoles and dynamic tubules as well as those emerging from the vacuole toward the cell periphery. - Information by UniProt
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Database links
- Entrez Gene: 8934 Human
- Omim: 603949 Human
- SwissProt: O14966 Human
- Unigene: 115325 Human
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Alternative names
- DKFZp686P1051 antibody
- Rab 7 like protein 1 antibody
- RAB 7L antibody
see all
Images
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All lanes : Anti-RAB29 (phospho S72) antibody [MJF-R40-R3-H3] (ab291075) at 1/500 dilution
Lane 1 : HEK-293T (human embryonic kidney epithelial cell) transfected with a human LRRK2[R1441G] expression vector and a human RAB29 expression vector containing an HA-tag, whole cell lysate
Lane 2 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 expression vector containing an HA-tag and treated with 200 µM MLi-2 for 1.5h, whole cell lysate,
Lane 3 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (T71A mutation) expression vector containing an HA-tag, whole cell lysate.
Lane 4 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (T71A mutation) expression vector containing an HA-tag and treated with 200 µM MLi-2 for 1.5h, whole cell lysate
Lane 5 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (S72A mutation) expression vector containing an HA-tag, whole cell lysate
Lane 6 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (S72A mutation) expression vector containing an HA-tag and treated with 200 µM MLi-2 for 1.5h, whole cell lysate
Lane 7 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (T71A and S72A mutation) expression vector containing an HA-tag, whole cell lysate
Lane 8 : HEK-293T transfected with a human LRRK2[R1441G] expression vector and a human RAB29 (T71A and S72A mutation) expression vector containing an HA-tag and treated with 200 µM MLi-2 for 1.5h, whole cell lysate
Lysates/proteins at 38 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
All the lysates were kindly provided by Prof. Dario Alessi, University of Dundee.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab291075 has not yet been referenced specifically in any publications.