Overview

  • Product name

    Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1]
    See all RAB29 primary antibodies
  • Description

    Rabbit monoclonal [MJF-R24-17-1] to RAB29 (phospho T71)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RAB29 aa 50-150 (phospho T71). The exact sequence is proprietary.
    Database link: O14966

  • Positive control

    • WB: HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C] whole cell lysate.
  • General notes

    This antibody was developed with support from The Michael J. Fox Foundation.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    MJF-R24-17-1
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    Rab GTPase key regulator in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans-Golgi network structure. Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Post-translational
    modifications

    In case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells (PubMed:22042847).
  • Cellular localization

    Cell membrane. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network. Vacuole. Cytoplasm, cytoskeleton. Colocalizes with LRRK2 along tubular structures emerging from Golgi apparatus (By similarity). Colocalizes with GM130 at the Golgi apparatus. Colocalizes with dynamic tubules emerging from and retracting to the Golgi apparatus. Colocalizes with TGN46 at the trans-Golgi network (TGN). In Salmonella enterica serovar Typhi (S.Typhi) infected epithelial cells, is recruited and colocalized with both S.Typhi-containing vacuoles and dynamic tubules as well as those emerging from the vacuole toward the cell periphery.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686P1051 antibody
    • Rab 7 like protein 1 antibody
    • RAB 7L antibody
    • Rab-7-like protein 1 antibody
    • RAB29 antibody
    • Rab7 like protein 1 antibody
    • RAB7 member RAS oncogene family like 1 antibody
    • RAB7L antibody
    • RAB7L_HUMAN antibody
    • Ras related protein Rab 7L1 antibody
    • Ras related protein Rab7L1 antibody
    • Ras-related protein Rab-29 antibody
    • Ras-related protein Rab-7L1 antibody
    see all

Images

  • Dot blot analysis of RAB29 (phospho T71) labeled with ab241062 at 1/1000 dilution.

    Lane 1: RAB29 (phospho T71) peptide.
    Lane 2: RAB29 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB3 and LRRK2 [Y1699C], whole cell lysate
    Lane 2 : HEK-293 overexpressing HA-tagged RAB5 and LRRK2 [Y1699C], whole cell lysate
    Lane 3 : HEK-293 overexpressing HA-tagged RAB8 and LRRK2 [Y1699C], whole cell lysate
    Lane 4 : HEK-293 overexpressing HA-tagged RAB10 and LRRK2 [Y1699C], whole cell lysate
    Lane 5 : HEK-293 overexpressing HA-tagged RAB12 and LRRK2 [Y1699C], whole cell lysate
    Lane 6 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate
    Lane 7 : HEK-293 overexpressing HA-tagged RAB35 and LRRK2 [Y1699C], whole cell lysate
    Lane 8 : HEK-293 overexpressing HA-tagged RAB43 and LRRK2 [Y1699C], whole cell lysate

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% BSA/TBST.

    The blot was scanned with LI-COR Odyssey CLx.

    This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

  • All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/500 dilution

    Lane 1 : MLi-2 (100nM for 60min) treated HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
    Lane 2 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
    Lane 3 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 1 µg
    Lane 4 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.3 µg
    Lane 5 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.1 µg

    Secondary
    All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking buffer:  5% NFDM/TBST.

    Dilution buffer: 5% BSA/TBST.

    The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates the phosphorylation of Rab proteins. While MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).

    The blot was scanned with LI-COR Odyssey CLx.

    This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee

References

ab241062 has not yet been referenced specifically in any publications.

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