Recombinant Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
![Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)](https://www.abcam.com/ps/products/241/ab241062/Images/ab241062-465436-anti-rab29-phospho-t71-antibody-mjf-r24-17-1-westernblot-human.jpg "Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R24-17-1] to RAB29 (phospho T71)
- Suitable for: Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1]
See all RAB29 primary antibodies -
Description
Rabbit monoclonal [MJF-R24-17-1] to RAB29 (phospho T71) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C] whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This antibody was developed with support from The Michael J. Fox Foundation.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R24-17-1 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
1/1000.
|
|
| WB |
1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
|
| Notes |
|---|
|
Dot blot
1/1000. |
|
WB
1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
Target
-
Function
Rab GTPase key regulator in vesicle trafficking. Essential for maintaining the integrity of the endosome-trans-Golgi network structure. Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Post-translational
modificationsIn case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells (PubMed:22042847). -
Cellular localization
Cell membrane. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Golgi apparatus, trans-Golgi network. Vacuole. Cytoplasm, cytoskeleton. Colocalizes with LRRK2 along tubular structures emerging from Golgi apparatus (By similarity). Colocalizes with GM130 at the Golgi apparatus. Colocalizes with dynamic tubules emerging from and retracting to the Golgi apparatus. Colocalizes with TGN46 at the trans-Golgi network (TGN). In Salmonella enterica serovar Typhi (S.Typhi) infected epithelial cells, is recruited and colocalized with both S.Typhi-containing vacuoles and dynamic tubules as well as those emerging from the vacuole toward the cell periphery. - Information by UniProt
-
Database links
- Entrez Gene: 8934 Human
- Omim: 603949 Human
- SwissProt: O14966 Human
- Unigene: 115325 Human
-
Alternative names
- DKFZp686P1051 antibody
- Rab 7 like protein 1 antibody
- RAB 7L antibody
see all
Images
-
Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)All lanes : Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062) at 1/5000 dilution
Lane 1 : HEK-293 (human embryonic kidney) over-expressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate
Lane 2 : MLi-2 (100nM for 60min) treated HEK-293 over-expressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaBlocking buffer: 5% NFDM/TBST.
-
![Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)](https://www.abcam.com/ps/products/241/ab241062/Images/ab241062-332542-anti-rab29-phospho-t71-antibody-mjf-r24-17-1-dot-blot.jpg)
Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)Dot blot analysis of RAB29 (phospho T71) labeled with ab241062 at 1/1000 dilution.
Lane 1: RAB29 (phospho T71) peptide.
Lane 2: RAB29 non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
![Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)](https://www.abcam.com/ps/products/241/ab241062/Images/ab241062-332579-anti-rab29-phospho-t71-antibody-mjf-r24-17-1-western-blot.jpg)
Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)All lanes : Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB3 and LRRK2 [Y1699C], whole cell lysate
Lane 2 : HEK-293 overexpressing HA-tagged RAB5 and LRRK2 [Y1699C], whole cell lysate
Lane 3 : HEK-293 overexpressing HA-tagged RAB8 and LRRK2 [Y1699C], whole cell lysate
Lane 4 : HEK-293 overexpressing HA-tagged RAB10 and LRRK2 [Y1699C], whole cell lysate
Lane 5 : HEK-293 overexpressing HA-tagged RAB12 and LRRK2 [Y1699C], whole cell lysate
Lane 6 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate
Lane 7 : HEK-293 overexpressing HA-tagged RAB35 and LRRK2 [Y1699C], whole cell lysate
Lane 8 : HEK-293 overexpressing HA-tagged RAB43 and LRRK2 [Y1699C], whole cell lysate
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% BSA/TBST.
The blot was scanned with LI-COR Odyssey CLx.
This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.
-
![Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)](https://www.abcam.com/ps/products/241/ab241062/Images/ab241062-332580-anti-rab29-phospho-t71-antibody-mjf-r24-17-1-western-blot.jpg)
Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)All lanes : Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062) at 1/500 dilution
Lane 1 : MLi-2 (100nM for 60min) treated HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
Lane 2 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
Lane 3 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 1 µg
Lane 4 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.3 µg
Lane 5 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.1 µg
Secondary
All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% BSA/TBST.
The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates the phosphorylation of Rab proteins. While MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).
The blot was scanned with LI-COR Odyssey CLx.
This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee
-
![Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)](https://www.abcam.com/ps/products/241/ab241062/Images/ab241062-6-benefits-of-recombinant-antibodies.png)
Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab241062 has been referenced in 1 publication.
- Gomez RC et al. Membrane association but not identity is required for LRRK2 activation and phosphorylation of Rab GTPases. J Cell Biol 218:4157-4170 (2019). PubMed: 31624137